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Dataset ID

NHA000133

Type of data
Loops from NGS (Hi-C)
Access criteria
Unrestricted-access
Total data volume
1.4 MB
File formats
  • XLSX
  • ZIP
Research
hum0207
Date published
2020-01-06
Date modified
2020-01-06
Secondary ID
hum0207.v1.HiC.v1

Unrestricted-access files linked to this dataset

FileLabelSizeCopy URL
hum0207.v1.HiC.v1.zip1.4 MB
hum0207.v1_header.xlsxDictionary file17.0 KB

Analysis method

Hi-C

Materials and participants
rheumatoid arthritis (ICD10: M05): 7 case
3 conditions of synovial fibroblasts (unstimulated, TNF-α, and 8-mix) were prepared for 1 case each. Samples under the same condition were pooled : total 3 samples
  • Health status
    Affected
  • Subject count
    7 (Individual)
Disease
rheumatoid arthritis (M05)
Sample description
DNAs extracted from pooled cells from rheumatoid arthritis patient
  • Tissue
    Synovial fibroblast
  • Tumor / normal
    Normal
Sample provider
N/A
Experimental method
Hi-C
Target
N/A
Reagent kit
Nextera Mate Pair Sample Preparation Kit
Fragmentation
Ultrasonic fragmentation (Covaris S2)
Platform
Illumina HiSeq
Read type
Paired-end
Read length
150 bp
Reference genome
GRCh37
Mapping
BWA-mem
QC and filtering
performed at "juicer" software
Analysis method
HiCCUPS
Read count
370 million - 400 million
Coverage (depth)
N/A
Coverage (breadth)
N/A
Processed data type
Hi-C loops