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Due to system maintenance, the application system, application review by the Data Access Committee will be unavailable during the following period.
Schedule: October 5th (Mon), 2026, 9:00 - October 7th (Wed), 2026, 15:00 (JST)
We apologize for any inconvenience this may cause and appreciate your understanding.

We are currently receiving a large number of applications for data submission, and the review process is taking longer than usual.We sincerely apologize for the delay and kindly ask for your understanding. When submitting an application, we would greatly appreciate it if you could allow sufficient time for the processing.

Following a change to our organizational structure effective April 1, 2026, this division has been renamed from the "Database Center for Life Science, Joint Support-Center for Data Science Research" to the "Database Division for Life Science (DBCLS), BioData Science Initiative (BSI), National Institute of Genetics (NIG)". Where the former name still appears in the guidelines, please read it as the new name.

Dataset ID

JGAD001047

Type of data
NGS (ChIP-seq)
Access criteria
Controlled-access (Type I)
Total data volume
38.0 GB
File formats
  • FASTQ
  • BED
Research
hum0556
Date published
2026-06-24
Date modified
2026-06-24
DDBJ Search
JGAD001047 (opens in a new tab)
JGA Study
JGAS000903 (opens in a new tab)

Analysis method

ChIP-seq

Materials and participants
lung cancer (ICD10: C349): 17 cases
tumor tissue: 24 samples (FFPE samples)
  • Health status
    Affected
  • Subject count
    17 (Individual)
Disease
lung cancer (C349)
Sample description
DNA extracted from tumor tissues was used for the library construction. Anti-histone antibody (H3K27ac) was used for immunoprecipitation.
  • Tumor / normal
    Tumor
Sample provider
N/A
Experimental method
ChIP-seq
Target
N/A
Reagent kit
ThruPLEX DNA-Seq Kit
Fragmentation
Ultrasonic fragmentation (Bioruptor)
Platform
Illumina HiSeq 2500
Read type
Single-end
Read length
50 bp
Reference genome
GRCh37
Mapping
Bowtie2
QC and filtering
- Read trimming using Trimmomatic (removal of TruSeq3 adapters; trimming when the average quality score across a 4-base sliding window falls below 20; removal of reads shorter than 36 bp)
- Post-mapping filtering using Samtools (removal of reads with MAPQ < 20)
Analysis method
MACS2
Data use policy
NBDC data sharing policy (JGAP000001)