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Due to system maintenance, the application system, application review by the Data Access Committee will be unavailable during the following period.
Schedule: October 5th (Mon), 2026, 9:00 - October 7th (Wed), 2026, 15:00 (JST)
We apologize for any inconvenience this may cause and appreciate your understanding.

We are currently receiving a large number of applications for data submission, and the review process is taking longer than usual.We sincerely apologize for the delay and kindly ask for your understanding. When submitting an application, we would greatly appreciate it if you could allow sufficient time for the processing.

Following a change to our organizational structure effective April 1, 2026, this division has been renamed from the "Database Center for Life Science, Joint Support-Center for Data Science Research" to the "Database Division for Life Science (DBCLS), BioData Science Initiative (BSI), National Institute of Genetics (NIG)". Where the former name still appears in the guidelines, please read it as the new name.

Dataset ID

JGAD001020

Type of data
NGS (FLeCS-seq, SMART-seq, RNA-seq, short-read WGS, ultralong-read WGS)
Access criteria
Controlled-access (Type I)
Total data volume
753 GB
File formats
  • FASTQ
  • BAM
Research
hum0006
Date published
2026-06-04
Date modified
2026-06-04
DDBJ Search
JGAD001020 (opens in a new tab)
JGA Study
JGAS000877 (opens in a new tab)

Analysis method

FLeCS-seq

Materials and participants
glioma (ICD10: C71): 1 case (2 samples)
  • Health status
    Affected
  • Subject count
    1 (Individual)
Disease
glioma (C71)
Sample description
RNAs extracted from normal left frontal lobe tissue
  • Tissue
    Brain
Sample provider
N/A
Experimental method
FLeCS-seq
Target
N/A
Reagent kit
Ligation Sequencing Kit (SQK-LSK114)
Fragmentation
N/A
Platform
Oxford Nanopore Technologies PromethION
Read type
Single-end
Read length
N/A
Data use policy
NBDC data sharing policy (JGAP000001)

SMART-seq

Materials and participants
glioma (ICD10: C71): 1 case (1 sample)
  • Health status
    Affected
  • Subject count
    1 (Individual)
Disease
glioma (C71)
Sample description
RNAs extracted from normal left frontal lobe tissue
  • Tissue
    Brain
Sample provider
N/A
Experimental method
SMART-seq
Target
N/A
Reagent kit
Ligation Sequencing Kit (SQK-LSK114)
SMART-Seq mRNA Long Read
Fragmentation
N/A
Platform
Oxford Nanopore Technologies PromethION
Read type
Single-end
Read length
N/A
Data use policy
NBDC data sharing policy (JGAP000001)

RNA-seq

Materials and participants
glioma (ICD10: C71): 1 case (2 samples)
  • Health status
    Affected
  • Subject count
    1 (Individual)
Disease
glioma (C71)
Sample description
RNAs extracted from normal left frontal lobe tissue
  • Tissue
    Brain
Sample provider
N/A
Experimental method
RNA-seq
Target
N/A
Reagent kit
SMART-Seq Stranded Kit
Fragmentation
Heat treatment in the presence of Mg²⁺
Platform
Element AVITI
Read type
Paired-end
Read length
300 bp
Data use policy
NBDC data sharing policy (JGAP000001)

Short-read WGS

Materials and participants
glioma (ICD10: C71): 1 case (1 sample)
  • Health status
    Affected
  • Subject count
    1 (Individual)
Disease
glioma (C71)
Sample description
RNAs extracted from normal left frontal lobe tissue
  • Tissue
    Brain
Sample provider
N/A
Experimental method
WGS
Target
N/A
Fragmentation
Ultrasonic fragmentation
Platform
Illumina NovaSeq 6000
Read type
Paired-end
Read length
300 bp
Data use policy
NBDC data sharing policy (JGAP000001)

Ultralong-read WGS

Materials and participants
glioma (ICD10: C71): 1 case (1 sample)
  • Health status
    Affected
  • Subject count
    1 (Individual)
Disease
glioma (C71)
Sample description
DNAs extracted from normal left frontal lobe tissue
  • Tissue
    Brain
Sample provider
N/A
Experimental method
WGS
Target
N/A
Reagent kit
Monarch HMW DNA Extraction Kit for Tissue
Ultra-Long DNA Sequencing Kit (SQK-ULK001)
Fragmentation
N/A
Platform
Oxford Nanopore Technologies PromethION
Read type
Single-end
Read length
N/A
Reference genome
GRCh38
Mapping
minimap2 (Dorado)
Mapping quality
Mapping rate 98.6%
Coverage (depth)
44x
Data use policy
NBDC data sharing policy (JGAP000001)