Dataset ID
JGAD000997
- Type of data
- NGS (Exome)
NGS (RNA-seq)
NGS (scRNA-seq)
NGS (Visium Spatial Gene Expression), Histological image
Xenium In Situ Gene Expression - Access criteria
- Controlled-access (Type I)
- Total data volume
- 1.0 TB
- File formats
- FASTQ
- BAM
- TSV
- PARQUET
- MTX
- HDF5
- TIFF
- Research
- hum0550
- Date published
- 2025-11-14
- Date modified
- 2025-11-14
- DDBJ Search
- JGAD000997 (opens in a new tab)
- JGA Study
- JGAS000855 (opens in a new tab)
Analysis method
WES
- Materials and participants
- renal cancer (ICD10: C64): 54 cases
tumor tissues: 125 samples
non-tumor tissues (cyst): 36 samples - Health statusAffected
- Subject count54 (Individual)
- Disease
- renal cancer (C64)
- Sample description
- DNAs extracted from tumor tissues and non-tumor tissues
- TissueCyst
- Tumor / normalMixed
- Sample provider
- N/A
- Experimental method
- WES
- Target
- N/A
- Reagent kit
- Twist Library Preparation Kit
- Fragmentation
- included in the above library construction kit
- Platform
- Illumina NovaSeq 6000
- Read type
- Paired-end
- Read length
- 150 bp
- Reference genome
- GRCh38
- Mapping
- BWA-MEM
- Mapping quality
- MAPQ > 20
- Coverage (depth)
- 127x
- Data use policy
- NBDC data sharing policy (JGAP000001)
RNA-seq
- Materials and participants
- renal cancer (ICD10: C64): 91 cases
tumor tissues: 91 samples - Health statusAffected
- Subject count91 (Individual)
- Disease
- renal cancer (C64)
- Sample description
- RNAs extracted from tumor tissues
- Tumor / normalTumor
- Sample provider
- N/A
- Experimental method
- RNA-seq
- Target
- N/A
- Reagent kit
- NEBNext Ultra Directional RNA Library Prep Kit for Illumina
- Fragmentation
- included in the above library construction kit
- Platform
- Illumina NovaSeq 6000
- Read type
- Paired-end
- Read length
- 150 bp
- Data use policy
- NBDC data sharing policy (JGAP000001)
scRNA-seq
- Materials and participants
- renal cancer (ICD10: C64): 18 cases
tumor tissues: 24 samples - Health statusAffected
- Subject count18 (Individual)
- Disease
- renal cancer (C64)
- Sample description
- RNAs extracted from tumor tissues
- Tumor / normalTumor
- Sample provider
- N/A
- Experimental method
- scRNA-seq
- Target
- N/A
- Reagent kit
- Chromium Next GEM Single Cell 3' v3.1
- Fragmentation
- included in the above library construction kit
- Platform
- Illumina NovaSeq 6000
- Read type
- Paired-end
- Read length
- read1: 28 bp
read2: 90 bp - Data use policy
- NBDC data sharing policy (JGAP000001)
Xenium 空間トランスクリプトーム
- Materials and participants
- renal cancer (ICD10: C64): 3 cases
tumor tissues: 4 samples - Health statusAffected
- Subject count3 (Individual)
- Disease
- renal cancer (C64)
- Sample description
- tumor tissues
- Tumor / normalTumor
- Sample provider
- N/A
- Experimental method
- Spatial transcriptomics
- Target
- N/A
- Reagent kit
- Xenium Human Multi-Tissue and Cancer Panel
Xenium In Situ Gene Expression - Platform
- 10x Genomics Xenium Analyzer
- Analysis method
- Xenium Analyzer
- Data use policy
- NBDC data sharing policy (JGAP000001)
Visium 空間トランスクリプトーム + 病理画像
- Materials and participants
- renal cancer (ICD10: C64): 16 cases
tumor tissues: 22 samples - Health statusAffected
- Subject count16 (Individual)
- Disease
- renal cancer (C64)
- Sample description
- tumor tissues
- Tumor / normalTumor
- Sample provider
- N/A
- Experimental method
- Histological image
Spatial transcriptomics - Target
- N/A
- Sample processing
- Histological staining: hematoxylin-eosin staining
- Reagent kit
- Visium Spatial Gene Expression
- Fragmentation
- included in the above library construction kit
- Platform
- Illumina NovaSeq 6000
- Read type
- Paired-end
- Read length
- read1: 28 bp
read2: 50 bp - Reference genome
- GRCh38
- Analysis method
- Space Ranger Pipeline (version 1.3.1)
- Data use policy
- NBDC data sharing policy (JGAP000001)