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We are currently receiving a large number of applications for data submission, and the review process is taking longer than usual.We sincerely apologize for the delay and kindly ask for your understanding. When submitting an application, we would greatly appreciate it if you could allow sufficient time for the processing.

Following a change to our organizational structure effective April 1, 2026, this division has been renamed from the "Database Center for Life Science, Joint Support-Center for Data Science Research" to the "Database Division for Life Science (DBCLS), BioData Science Initiative (BSI), National Institute of Genetics (NIG)". Where the former name still appears in the guidelines, please read it as the new name.

Dataset ID

JGAD000969

Type of data
NGS (scRNA-seq, scBCR-seq, scTCR-seq, CITE-seq)
Access criteria
Controlled-access (Type I)
Total data volume
1.2 TB
File formats
  • FASTQ
  • CSV
Research
hum0521
Date published
2025-09-01
Date modified
2025-09-01
DDBJ Search
JGAD000969 (opens in a new tab)
JGA Study
JGAS000827 (opens in a new tab)

Analysis method

scRNA-seq

Materials and participants
uterine cancer (ICD10: C549): 10 cases
B cells isolated from tumor tissues: 10 cases, 11 samples
(one of them acquired scRNA-seq data twice)
T cells isolated from tumor tissues: 5 cases, 5 samples
hereditary breast and ovarian cancer syndrome (ICD10: R798): 1 case
B cells isolated from non-tumor tissues: 1 case, 1 sample
T cells isolated from non-tumor tissues: 1 case, 1 sample
  • Health status
    Affected
  • Subject count
    18 (Sample)
Disease
  • uterine cancer
  • hereditary breast and ovarian cancer syndrome
Sample description
B and T cells isolated from uterine tissue
  • Tissue
    B cell, T cell, Uterus
  • Tumor / normal
    Mixed
Sample provider
N/A
Experimental method
scRNA-seq
Target
N/A
Reagent kit
Chromium Next GEM Single Cell 3' v3.1
Chromium Next GEM Single Cell 5' v2
Fragmentation
included in the above library construction kit
Platform
Illumina NovaSeq 6000
Read type
Paired-end
Read length
N/A
Reference genome
GRCh38
Mapping
Cell Ranger v5.0.1
QC and filtering
Cell Ranger v5.0.1
Analysis method
Cell Ranger v5.0.1
Gene count
N/A
Data use policy
NBDC data sharing policy (JGAP000001)

scBCR-seq

Materials and participants
uterine cancer (ICD10: C549): 4 cases
B cells isolated from tumor tissues: 4 cases, 4 samples
hereditary breast and ovarian cancer syndrome (ICD10: R798): 1 case
B cells isolated from non-tumor tissues: 1 case, 1 sample
  • Health status
    Affected
  • Subject count
    5 (Individual)
Disease
  • uterine cancer
  • hereditary breast and ovarian cancer syndrome
Sample description
B cells isolated from uterine tissue
  • Tissue
    B cell, Uterus
  • Tumor / normal
    Mixed
Sample provider
N/A
Experimental method
BCR-seq
Target
N/A
Reagent kit
Chromium Next GEM Single Cell 5' v2
Fragmentation
included in the above library construction kit
Platform
Illumina NovaSeq 6000
Read type
Paired-end
Read length
N/A
Reference genome
GRCh38
Mapping
Cell Ranger v5.0.1
QC and filtering
Cell Ranger v5.0.1
Analysis method
Cell Ranger v5.0.1
Data use policy
NBDC data sharing policy (JGAP000001)

scTCR-seq

Materials and participants
uterine cancer (ICD10: C549): 5 cases
T cells isolated from tumor tissues: 5 cases, 5 samples
hereditary breast and ovarian cancer syndrome (ICD10: R798): 1 case
T cells isolated from non-tumor tissues: 1 case, 1 sample
  • Health status
    Affected
  • Subject count
    6 (Individual)
Disease
  • uterine cancer
  • hereditary breast and ovarian cancer syndrome
Sample description
T cells isolated from uterine tissue
  • Tissue
    T cell, Uterus
  • Tumor / normal
    Mixed
Sample provider
N/A
Experimental method
TCR-seq
Target
N/A
Reagent kit
Chromium Next GEM Single Cell 5' v2
Fragmentation
included in the above library construction kit
Platform
Illumina NovaSeq 6000
Read type
Paired-end
Read length
N/A
Reference genome
GRCh38
Mapping
Cell Ranger v5.0.1
QC and filtering
Cell Ranger v5.0.1
Analysis method
Cell Ranger v5.0.1
Data use policy
NBDC data sharing policy (JGAP000001)

CITE-seq

Materials and participants
uterine cancer (ICD10: C549): 6 cases
B cells isolated from tumor tissues: 6 cases, 6 samples
T cells isolated from tumor tissues: 5 cases, 5 samples
  • Health status
    Affected
  • Subject count
    11 (Sample)
Disease
  • uterine cancer
Sample description
B and T cells isolated from uterine tissue
  • Tissue
    B cell, T cell, Uterus
  • Tumor / normal
    Tumor
Sample provider
N/A
Experimental method
CITE-seq
Target
N/A
Reagent kit
Chromium Next GEM Single Cell 3' v3.1
Chromium Next GEM Single Cell 5' v2
TotalSeq-B
TotalSeq-C
Fragmentation
included in the above library construction kit
Platform
Illumina NovaSeq 6000
Read type
Paired-end
Read length
N/A
Reference genome
N/A
Mapping
Cell Ranger v5.0.1
QC and filtering
Cell Ranger v5.0.1
Analysis method
Cell Ranger v5.0.1
Data use policy
NBDC data sharing policy (JGAP000001)