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Due to system maintenance, the application system, application review by the Data Access Committee will be unavailable during the following period.
Schedule: October 5th (Mon), 2026, 9:00 - October 7th (Wed), 2026, 15:00 (JST)
We apologize for any inconvenience this may cause and appreciate your understanding.

We are currently receiving a large number of applications for data submission, and the review process is taking longer than usual.We sincerely apologize for the delay and kindly ask for your understanding. When submitting an application, we would greatly appreciate it if you could allow sufficient time for the processing.

Following a change to our organizational structure effective April 1, 2026, this division has been renamed from the "Database Center for Life Science, Joint Support-Center for Data Science Research" to the "Database Division for Life Science (DBCLS), BioData Science Initiative (BSI), National Institute of Genetics (NIG)". Where the former name still appears in the guidelines, please read it as the new name.

Dataset ID

JGAD000957

Type of data
NGS
(scRNA-seq, scTCR-seq, scCITE-seq, bulk RNA-seq)
Access criteria
Controlled-access (Type I)
Total data volume
1.1 TB
File formats
  • FASTQ
  • TXT
  • TAR
Research
hum0511
Date published
2026-08-03
Date modified
2026-08-03
DDBJ Search
JGAD000957 (opens in a new tab)
JGA Study
JGAS000815 (opens in a new tab)

Unrestricted-access files linked to this dataset

Download the URL list
FileLabelSizeCopy URL
commitment_hum0511.pdf733 KB
commitment_hum0511_e.pdf149 KB

Analysis method

scRNA-seq

Materials and participants
Dataset 1: 28 healthy individuals
70s-90s: 8
100s: 10
110s: 10
Dataset 2: 6 healthy individuals included in Dataset 1
70s-90s: 2
100s: 2
110s: 2
  • Health status
    Healthy
  • Subject count
    28 (Individual)
  • Age group
    Elderly
Sample description
Dataset 1: RNAs extracted from peripheral blood T cells
Dataset 2: RNAs extracted from peripheral blood T cells stimulated for 6 hours with PMA and ionomycin
  • Tissue
    Peripheral blood, T cell
Experimental method
scRNA-seq
Reagent kit
Chromium Next GEM Single Cell 5' v2
Fragmentation
Enzymatic fragmentation
Platform
MGI DNBSEQ-G400
Read type
Paired-end
Read length
72 bp (Read1)
91 bp (Read2)
Reference genome
GRCh38
Mapping
Cell Ranger 6.1.2
QC and filtering
Cell Ranger 6.1.2
Analysis method
Cell Ranger 6.1.2
Data use policy
JGAP000013 Policy
NBDC data sharing policy (JGAP000001)

scTCR-seq

Materials and participants
Dataset 1: 28 healthy individuals
70s-90s: 8
100s: 10
110s: 10
Dataset 2: 6 healthy individuals included in Dataset 1
70s-90s: 2
100s: 2
110s: 2
  • Health status
    Healthy
  • Subject count
    28 (Individual)
  • Age group
    Elderly
Sample description
Dataset 1: RNAs extracted from peripheral blood T cells
Dataset 2: RNAs extracted from peripheral blood T cells stimulated for 6 hours with PMA and ionomycin
  • Tissue
    Peripheral blood, T cell
  • Tumor / normal
    Normal
Experimental method
TCR-seq
Reagent kit
Chromium Single Cell V(D)J Enrichment Kit, Human T Cell
Fragmentation
Enzymatic fragmentation
Platform
MGI DNBSEQ-G400
Read type
Paired-end
Read length
72 bp (Read1)
91 bp (Read2)
Reference genome
GRCh38
Mapping
Cell Ranger 6.1.2
QC and filtering
Cell Ranger 6.1.2
Analysis method
Cell Ranger 6.1.2
Data use policy
JGAP000013 Policy
NBDC data sharing policy (JGAP000001)

scCITE-seq

Materials and participants
Dataset 1: 28 healthy individuals
70s-90s: 8
100s: 10
110s: 10
Dataset 2: 6 healthy individuals included in Dataset 1
70s-90s: 2
100s: 2
110s: 2
  • Health status
    Healthy
  • Subject count
    28 (Individual)
  • Age group
    Elderly
Sample description
Dataset 1: RNAs extracted from peripheral blood T cells
Dataset 2: RNAs extracted from peripheral blood T cells stimulated for 6 hours with PMA and ionomycin
  • Tissue
    Peripheral blood, T cell
  • Tumor / normal
    Normal
Experimental method
CITE-seq
Target
CD3, CD4, CD8, CD25, CD27, CD28, CD45RA, CD45RO, CD95, TCRD, CCR7, IL7RA, PD-1, CD161, KLRG1, CD56
Reagent kit
Chromium Single Cell 5' Feature Barcode Library Kit
Fragmentation
Enzymatic fragmentation
Platform
MGI DNBSEQ-G400
Read type
Paired-end
Read length
0 bp (Read1)
15 bp (Read2)
Reference genome
N/A
Mapping
Cell Ranger 6.1.2
QC and filtering
Cell Ranger 6.1.2
Analysis method
Cell Ranger 6.1.2
Data use policy
JGAP000013 Policy
NBDC data sharing policy (JGAP000001)

bulk RNA-seq

Materials and participants
1 healthy individual included in Dataset1
100s: 1
  • Health status
    Healthy
  • Subject count
    1 (Individual)
  • Population
    Japanese
  • Age group
    Elderly
Sample description
RNAs extracted from CD4⁺CD27⁻CD28⁻ T cells and CD4⁺CD45RO⁺CD28⁺ T cells sorted from peripheral blood mononuclear cells
  • Tissue
    Peripheral blood, T cell
  • Tumor / normal
    Normal
Experimental method
RNA-seq
Target
N/A
Reagent kit
Nextera XT DNA Library Prep Kit
Fragmentation
Enzymatic fragmentation
Platform
Illumina NovaSeq X
Read type
Paired-end
Read length
151 bp (Read1)
151 bp (Read2)
Data use policy
JGAP000013 Policy
NBDC data sharing policy (JGAP000001)