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Dataset ID

JGAD000946

Type of data
NGS (WGS, RNA-seq, EM-seq)
Xenium In Situ Gene Expression
Access criteria
Controlled-access (Type I)
Total data volume
196 GB
File formats
  • FASTQ
  • TAR
Research
hum0200
Date published
2026-02-05
Date modified
2026-02-05

Unrestricted-access files linked to this dataset

FileLabelSizeCopy URL
gene_list.pdf379 genes188 KB

Analysis method

WGS

Materials and participants
IDC (ICD10: C509): 1 case
non-tumor tissue (normal breast tissue): 1 sample
  • Health status
    Affected
  • Subject count
    1 (Individual)
Disease
IDC (C509)
Sample description
DNAs extracted from tumor and non-tumor tissues
  • Tissue
    Breast
  • Tumor / normal
    Mixed
Sample provider
N/A
Experimental method
WGS
Target
N/A
Reagent kit
NEBNext Enzymatic Methyl-seq Kit
Fragmentation
Ultrasonic fragmentation (Covaris)
Platform
Illumina NovaSeq 6000
Read type
Paired-end
Read length
300 bp

RNA-seq

Materials and participants
IDC (ICD10: C509): 1 case
non-tumor tissue (normal breast tissue): 1 sample
  • Health status
    Affected
  • Subject count
    1 (Individual)
Disease
IDC (C509)
Sample description
RNAs extracted from tumor and non-tumor tissues
  • Tissue
    Breast
  • Tumor / normal
    Mixed
Sample provider
N/A
Experimental method
RNA-seq
Target
N/A
Reagent kit
SMART-Seq Stranded Kit
Fragmentation
Heat treatment with Shearing Master Mix
Platform
Illumina NovaSeq 6000
Read type
Paired-end
Read length
300 bp

EM-seq

Materials and participants
IDC (ICD10: C509): 1 case
non-tumor tissue (normal breast tissue): 1 sample
  • Health status
    Affected
  • Subject count
    1 (Individual)
Disease
IDC (C509)
Sample description
DNAs extracted from tumor and non-tumor tissues
  • Tissue
    Breast
  • Tumor / normal
    Mixed
Sample provider
N/A
Experimental method
EM-seq
Target
N/A
Reagent kit
NEBNext Enzymatic Methyl-seq Kit
Fragmentation
Ultrasonic fragmentation (Covaris)
Platform
Illumina NovaSeq 6000
Read type
Paired-end
Read length
300 bp

Xenium 空間トランスクリプトーム

Materials and participants
IDC (ICD10: C509): 1 case
tumor tissue: 3 samples (primary tumor, metastatic lympho node, tumor-draining lymph node)
  • Health status
    Affected
  • Subject count
    1 (Individual)
Disease
IDC (C509)
Sample description
FFPE tumor tissues
  • Tissue
    Breast, Lymph node
  • Tumor / normal
    Tumor
Sample provider
N/A
Experimental method
Spatial transcriptomics
Target
379 genes (Human Breast panel: 280 genes, custom: 99 genes)
Platform
10x Genomics Xenium Analyzer
Analysis method
xenium-1.7.1.0