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- Release date
- 2025-08-08
- Date modified
- 2025-08-08
- Research
- hum0497
- Type of data
- NGS (RNA-seq)
- Access type
- Controlled-access (Type I)
- JGA Study
- JGAS000777
Analysis method
RNA-seq
- Materials and Participants
human term placentas from three individuals (ICD10: O82.0, O80.0) trophoblast stem cell (Ch-TS) lines derived from the chorion: 3 samples differentiated extravillous trophoblast cells (Ch-EVT): 3 samples differentiated syncytiotrophoblast cells (Ch-ST): 3 samples human trophoblast stem cell lines from RIKEN BRC (CT27, CT29, bTS5) trophoblast stem cells: 3 samples differentiated extravillous trophoblast cells: 2 samples differentiated syncytiotrophoblast cells: 2 samples
- Experimental Method
RNA-seq
- Platform
Illumina [NovaSeq 6000]
- Sample Description
total RNA extracted from trophoblast stem cells, extravillous trophoblast cells and syncytiotrophoblast cells
- Cell Lines
RIKEN BRC (CT27 0RCB49361, CT29 2RCB49373, bTS5 4RCB49405)
- Library Construction
NEBNext Poly(A) mRNA Magnetic Isolation Module, NEBNext Ultra II Directional RNA Library Prep Kit, NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs)
- Fragmentation
chemical fragmentation at 94℃
- Read Type
Paired-end
- Read Length
100 bp
- Analysis Methods
featureCounts (GRCh38.106)
- Mapping
HISAT2
- Mapping Quality
Mapping rate>98%
- Reference Sequence
GRCh38/hg38
- Japanese Genotype-phenotype Archive Dataset Accession
- Total Data Volume
68.3 GB (fastq, csv)
- Policies