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We are currently receiving a large number of applications for data submission, and the review process is taking longer than usual.We sincerely apologize for the delay and kindly ask for your understanding. When submitting an application, we would greatly appreciate it if you could allow sufficient time for the processing.

Following a change to our organizational structure effective April 1, 2026, this division has been renamed from the "Database Center for Life Science, Joint Support-Center for Data Science Research" to the "Database Division for Life Science (DBCLS), BioData Science Initiative (BSI), National Institute of Genetics (NIG)". Where the former name still appears in the guidelines, please read it as the new name.

Dataset ID

JGAD000919

Type of data
NGS (RNA-seq)
Access criteria
Controlled-access (Type I)
Total data volume
47.3 GB
File formats
  • FASTQ
  • CSV
Research
hum0497
Date published
2025-08-08
Date modified
2025-08-08
DDBJ Search
JGAD000919 (opens in a new tab)
JGA Study
JGAS000777 (opens in a new tab)

Analysis method

RNA-seq

Materials and participants
human term placentas from three individuals (ICD10: O82.0, O80.0)
trophoblast stem cell (Ch-TS) lines derived from the chorion: 3 samples
differentiated extravillous trophoblast cells (Ch-EVT): 3 samples
differentiated syncytiotrophoblast cells (Ch-ST): 3 samples
human trophoblast stem cell lines from RIKEN BRC (CT27, CT29, bTS5)
trophoblast stem cells: 3 samples
differentiated extravillous trophoblast cells: 2 samples
differentiated syncytiotrophoblast cells: 2 samples
  • Health status
    Healthy
  • Subject count
    3 (Individual)
Disease
  • human term placentas
Sample description
total RNA extracted from trophoblast stem cells, extravillous trophoblast cells and syncytiotrophoblast cells
  • Tissue
    Chorion, Placenta
  • Tumor / normal
    Normal
Cell line
bTS5
CT27
CT29
Sample provider
RIKEN BRC (CT27 0RCB49361, CT29 2RCB49373, bTS5 4RCB49405)
Experimental method
RNA-seq
Target
N/A
Reagent kit
NEBNext Multiplex Oligos for Illumina
NEBNext Poly(A) mRNA Magnetic Isolation Module
NEBNext Ultra II Directional RNA Library Prep Kit for Illumina
Fragmentation
chemical fragmentation at 94℃
Platform
Illumina NovaSeq 6000
Read type
Paired-end
Read length
100 bp
Reference genome
GRCh38
Mapping
HISAT2
Mapping quality
Mapping rate>98%
Analysis method
featureCounts (GRCh38.106)
Data use policy
NBDC data sharing policy (JGAP000001)