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Due to system maintenance, the application system, application review by the Data Access Committee will be unavailable during the following period.
Schedule: October 5th (Mon), 2026, 9:00 - October 7th (Wed), 2026, 15:00 (JST)
We apologize for any inconvenience this may cause and appreciate your understanding.

We are currently receiving a large number of applications for data submission, and the review process is taking longer than usual.We sincerely apologize for the delay and kindly ask for your understanding. When submitting an application, we would greatly appreciate it if you could allow sufficient time for the processing.

Following a change to our organizational structure effective April 1, 2026, this division has been renamed from the "Database Center for Life Science, Joint Support-Center for Data Science Research" to the "Database Division for Life Science (DBCLS), BioData Science Initiative (BSI), National Institute of Genetics (NIG)". Where the former name still appears in the guidelines, please read it as the new name.

Dataset ID

JGAD000898

Type of data
NGS (WGS, t-nanoEM)
Access criteria
Controlled-access (Type I)
Total data volume
1.3 TB
File formats
  • FASTQ
  • BAM
Research
hum0394
Date published
2024-11-22
Date modified
2025-06-24
DDBJ Search
JGAD000898 (opens in a new tab)
JGA Study
JGAS000757 (opens in a new tab)

Analysis method

WGS

Materials and participants
non-small cell lung cancer (ICD10: C349): 1 + 1 cases
tumor tissue: 1 + 1 samples (Illumina, Nanopore)
non-tumor tissue: 1 + 1 samples (Illumina)
  • Health status
    Mixed
  • Subject count
    2 (Individual)
Disease
non-small cell lung cancer (C349)
Sample description
DNAs extracted from tumor tissue and non-tumor tissue
  • Tumor / normal
    Mixed
Sample provider
N/A
Experimental method
WGS
Target
N/A
Reagent kit
Ligation Sequencing Kit (SQK-LSK114)
NEBNext Multiplex Oligos for Illumina
NEBNext Ultra II DNA Library Prep Kit for Illumina
TruSeq Nano DNA Library Prep Kit
Fragmentation
Illumina: Ultrasonic fragmentation (Covaris)
Nanopore: -
Platform
Illumina NovaSeq 6000
Oxford Nanopore Technologies PromethION
Read type
Mixed
Read length
Illumina: 300 bp
Nanopore: 7500 bp (Mean)
Reference genome
GRCh38
Mapping
Illumina: BWA-MEM
Nanopore: Guppy
Mapping quality
N/A
Coverage (depth)
Tumor tissue: 82x (Illumina)
Non-tumor tissue: 44x (Illumina)
Data use policy
NBDC data sharing policy (JGAP000001)

t-nanoEM (Nanopore methylation)

Materials and participants
non-small cell lung cancer (ICD10: C349): 1 + 1 cases
multiple microdissected tumor regions: 1 + 1 samples
  • Health status
    Affected
  • Subject count
    2 (Individual)
Disease
non-small cell lung cancer (C349)
Sample description
DNA extracted from tumor tissue sample
  • Tumor / normal
    Tumor
Sample provider
N/A
Experimental method
t-nanoEM
Target
Twist Alliance Pan-cancer Methylation Panel - 1.5 MB
Reagent kit
NEBNext Enzymatic Methyl-seq Kit
Twist Standard Hyb and Wash Kit v2
Fragmentation
g-TUBE (Covaris)
Platform
Oxford Nanopore Technologies PromethION
Read type
Single-end
Read length
4800 bp (Mean)
Data use policy
NBDC data sharing policy (JGAP000001)