Dataset ID
JGAD000871
- Type of data
- NGS (scRNA-seq)
NGS (Visium Spatial Gene Expression), Histological image - Access criteria
- Controlled-access (Type I)
- Total data volume
- 806 GB
- File formats
- FASTQ
- TSV
- CSV
- JSON
- MTX
- HDF5
- PNG
- GZIP
- Research
- hum0476
- Date published
- 2025-01-08
- Date modified
- 2025-06-11
- DDBJ Search
- JGAD000871 (opens in a new tab)
- JGA Study
- JGAS000736 (opens in a new tab)
Analysis method
scRNA-seq
- Materials and participants
- renal cancer (ICD10: C64, N028, N088, N26, E142, N12): 32 cases
normal tissue: 3 samples
findings of IgA nephropathy: 3 samples
findings of early diabetic changes: 2 samples
findings of IgA deposition: 2 samples
findings of glomerular hyperplasia: 1 sample
findings of nephrosclerosis and diabetic nephropathy: 1 sample
findings of nephrosclerosis and IgA deposition: 1 sample
findings of diabetic nephropathy: 1 sample - Health statusAffected
- Subject count14 (Individual)
- Disease
- renal cancer (C64, N028, N088, N26, E142, N12)
- Sample description
- RNAs extracted from non-tumor tissues
- TissueKidney
- Tumor / normalNormal
- Sample provider
- N/A
- Experimental method
- scRNA-seq
- Target
- N/A
- Reagent kit
- Chromium Next GEM Single Cell 3' v3.1
- Fragmentation
- 10x Genomics's protocol
- Platform
- Illumina NextSeq 2000
- Read type
- Paired-end
- Read length
- Read 1: 28 bp
Read2: 90 bp - Reference genome
- GRCh38
- Mapping
- STAR
- QC and filtering
- N/A
- Analysis method
- Cell Ranger 4.0.0
- Gene count
- 53 (SC)
28,183 (Total genes detected)
2046 (Median genes per cell)
68 (SC)
28,837 (Total genes detected)
2665 (Median genes per cell)
136 (SC)
28,357 (Total genes detected)
2263 (Median genes per cell)
176 (SC)
29,154 (Total genes detected)
2588 (Median genes per cell)
204 (SC)
29,034 (Total genes detected)
2848 (Median genes per cell)
286 (SC)
28,351 (Total genes detected)
2680 (Median genes per cell) - Processed data type
- Gene expression matrix
- Phenotype data
- Included
- Data use policy
- NBDC data sharing policy (JGAP000001)
Visium 空間トランスクリプトーム + 病理画像
- Materials and participants
- renal cancer (ICD10: C64, N028, N088, N26, E142, N12): 32 cases
normal tissue: 9 samples
findings of IgA nephropathy: 6 samples
findings of early diabetic changes: 2 samples
findings of IgA deposition: 2 samples
findings of glomerular hyperplasia: 1 sample
findings of nephrosclerosis: 7 samples
findings of nephrosclerosis and diabetic nephropathy: 1 sample
findings of nephrosclerosis and IgA deposition: 2 samples
findings of diabetic nephropathy: 1 sample
findings of tubulointerstitial nephritis: 1 sample - Health statusAffected
- Subject count32 (Individual)
- Disease
- renal cancer (C64, N028, N088, N26, E142, N12)
- Sample description
- non-tumor samples from surgically resected tissues
- TissueKidney
- Tumor / normalNormal
- Sample provider
- N/A
- Experimental method
- Histological image
Spatial transcriptomics - Target
- N/A
- Sample processing
- Histological staining: hematoxylin-eosin staining
- Reagent kit
- Visium Human Transcriptome Probe Kit
Visium Spatial Gene Expression for FFPE - Fragmentation
- N/A
- Platform
- Illumina NovaSeq 6000
- Read type
- Paired-end
- Read length
- Read 1: 28 bp
Read2: 50 bp - Reference genome
- GRCh38
- Analysis method
- Space ranger, Seurat, Loupe
- Processed data type
- Histological image
Spatial gene expression data - Data use policy
- NBDC data sharing policy (JGAP000001)