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Dataset ID

JGAD000871

Type of data
NGS (scRNA-seq)
NGS (Visium Spatial Gene Expression), Histological image
Access criteria
Controlled-access (Type I)
Total data volume
806 GB
File formats
  • FASTQ
  • TSV
  • CSV
  • JSON
  • MTX
  • HDF5
  • PNG
  • GZIP
Research
hum0476
Date published
2025-01-08
Date modified
2025-06-11

Analysis method

scRNA-seq

Materials and participants
renal cancer (ICD10: C64, N028, N088, N26, E142, N12): 32 cases
normal tissue: 3 samples
findings of IgA nephropathy: 3 samples
findings of early diabetic changes: 2 samples
findings of IgA deposition: 2 samples
findings of glomerular hyperplasia: 1 sample
findings of nephrosclerosis and diabetic nephropathy: 1 sample
findings of nephrosclerosis and IgA deposition: 1 sample
findings of diabetic nephropathy: 1 sample
  • Health status
    Affected
  • Subject count
    14 (Individual)
Disease
renal cancer (C64, N028, N088, N26, E142, N12)
Sample description
RNAs extracted from non-tumor tissues
  • Tissue
    Kidney
  • Tumor / normal
    Normal
Sample provider
N/A
Experimental method
scRNA-seq
Target
N/A
Reagent kit
Chromium Next GEM Single Cell 3' v3.1
Fragmentation
10x Genomics's protocol
Platform
Illumina NextSeq 2000
Read type
Paired-end
Read length
Read 1: 28 bp
Read2: 90 bp
Reference genome
GRCh38
Mapping
STAR
QC and filtering
N/A
Analysis method
Cell Ranger 4.0.0
Gene count
53 (SC)
28,183 (Total genes detected)
2046 (Median genes per cell)
68 (SC)
28,837 (Total genes detected)
2665 (Median genes per cell)
136 (SC)
28,357 (Total genes detected)
2263 (Median genes per cell)
176 (SC)
29,154 (Total genes detected)
2588 (Median genes per cell)
204 (SC)
29,034 (Total genes detected)
2848 (Median genes per cell)
286 (SC)
28,351 (Total genes detected)
2680 (Median genes per cell)
Processed data type
Gene expression matrix
Phenotype data
Included

Visium 空間トランスクリプトーム + 病理画像

Materials and participants
renal cancer (ICD10: C64, N028, N088, N26, E142, N12): 32 cases
normal tissue: 9 samples
findings of IgA nephropathy: 6 samples
findings of early diabetic changes: 2 samples
findings of IgA deposition: 2 samples
findings of glomerular hyperplasia: 1 sample
findings of nephrosclerosis: 7 samples
findings of nephrosclerosis and diabetic nephropathy: 1 sample
findings of nephrosclerosis and IgA deposition: 2 samples
findings of diabetic nephropathy: 1 sample
findings of tubulointerstitial nephritis: 1 sample
  • Health status
    Affected
  • Subject count
    32 (Individual)
Disease
renal cancer (C64, N028, N088, N26, E142, N12)
Sample description
non-tumor samples from surgically resected tissues
  • Tissue
    Kidney
  • Tumor / normal
    Normal
Sample provider
N/A
Experimental method
Histological image
Spatial transcriptomics
Target
N/A
Sample processing
Histological staining: hematoxylin-eosin staining
Reagent kit
Visium Human Transcriptome Probe Kit
Visium Spatial Gene Expression for FFPE
Fragmentation
N/A
Platform
Illumina NovaSeq 6000
Read type
Paired-end
Read length
Read 1: 28 bp
Read2: 50 bp
Reference genome
GRCh38
Analysis method
Space ranger, Seurat, Loupe
Processed data type
Histological image
Spatial gene expression data