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Due to system maintenance, the application system, application review by the Data Access Committee will be unavailable during the following period.
Schedule: October 5th (Mon), 2026, 9:00 - October 7th (Wed), 2026, 15:00 (JST)
We apologize for any inconvenience this may cause and appreciate your understanding.

We are currently receiving a large number of applications for data submission, and the review process is taking longer than usual.We sincerely apologize for the delay and kindly ask for your understanding. When submitting an application, we would greatly appreciate it if you could allow sufficient time for the processing.

Following a change to our organizational structure effective April 1, 2026, this division has been renamed from the "Database Center for Life Science, Joint Support-Center for Data Science Research" to the "Database Division for Life Science (DBCLS), BioData Science Initiative (BSI), National Institute of Genetics (NIG)". Where the former name still appears in the guidelines, please read it as the new name.

Dataset ID

JGAD000793

Type of data
NGS (scRNA-seq, CUT＆RUN-seq)
Access criteria
Controlled-access (Type I)
Total data volume
474 GB
File formats
  • FASTQ
Research
hum0249
Date published
2025-04-14
Date modified
2025-04-14
DDBJ Search
JGAD000793 (opens in a new tab)
JGA Study
JGAS000663 (opens in a new tab)

Analysis method

scRNA-seq

Materials and participants
hard tissue/connective tissue related diseases (ICD10: Q870): 2 cases
iPSCs derived from oral mucosa of a fibroblast patient with Cleidocranial Dysostosis (CCD) (mutation-corrected iPS cells by gene editing) : 1 case (1 sample)
osteoblast differentiated from iPSCs derived from oral mucosa of a fibroblast patient with Cleidocranial Dysostosis (CCD) (mutation-corrected iPS cells by gene editing): 1 case (1 sample)
iPSCs derived from oral mucosa of a fibroblast patient with Cleidocranial Dysostosis (CCD) (RUNX2 knocked out iPS cells by gene editing): 1 case (1 sample)
osteoblast differentiated from iPSCs derived from oral mucosa of a fibroblast patient with Cleidocranial Dysostosis (CCD) (RUNX2 knocked out iPS cells by gene editing): 1 case (1 sample)
osteoblast differentiated from iPSCs derived from oral mucosa of a fibroblast patient with Cleidocranial Dysostosis (CCD) (RUNX2 knocked out iPS cells by gene editing) with forced expression of SYNE1: 1 case (1 sample)
  • Health status
    Affected
  • Subject count
    2 (Individual)
Disease
hard tissue/connective tissue related diseases (Q870)
Sample description
RNA extracted from iPS cells and osteoblast differentiated from iPS cells
  • Tissue
    Buccal mucosa
  • Tumor / normal
    Normal
Cell line
iPSC
Sample provider
N/A
Experimental method
scRNA-seq
Target
N/A
Reagent kit
Chromium Next GEM Single Cell 5' v2
Fragmentation
Enzymatic fragmentation
Platform
Illumina NovaSeq 6000
Read type
Paired-end
Read length
150 bp
Data use policy
NBDC data sharing policy (JGAP000001)

CUT&RUN-seq

Materials and participants
hard tissue/connective tissue related diseases (ICD10: Q870): 2 cases
osteoblast differentiated from iPS cells established from oral mucosa fibroblast: 2 cases (2 samples)
osteoblast differentiated from ips cells established from oral mucosa fibroblast (mutation-corrected ips cells by gene editing): 1 case (1 sample)
  • Health status
    Affected
  • Subject count
    2 (Individual)
Disease
hard tissue/connective tissue related diseases (Q870)
Sample description
DNA extracted from osteoblast differentiated from iPS cells
  • Tissue
    Buccal mucosa
  • Tumor / normal
    Normal
Sample provider
N/A
Experimental method
CUT&RUN-seq
Target
N/A
Reagent kit
CUT&RUN Library Prep Kit
Fragmentation
Enzymatic fragmentation
Platform
Illumina NovaSeq 6000
Read type
Paired-end
Read length
150 bp
Data use policy
NBDC data sharing policy (JGAP000001)