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Dataset ID

JGAD000773

Type of data
NGS (WGS, RNA-seq)
Access criteria
Controlled-access (Type I)
Total data volume
85.9 GB
File formats
  • FASTQ
Research
hum0429
Date published
2023-10-31
Date modified
2023-10-31

Analysis method

WGS

Materials and participants
tumor tissue collected from LU99 tumor-bearing mice: total 16 samples
tumor tissue collected from mice with treatment (JDQ443 + TNO155 + alpelisib + ribociclib) / vehicle condition at 3 hours / 24 hours after the last dose: 4 samples each
  • Health status
    Affected
  • Subject count
    24 (Sample)
Disease
human lung cancer (C349)
Sample description
DNAs extracted from tumor tissues
  • Tumor / normal
    Tumor
Cell line
LU99
Experimental method
WGS
Target
N/A
Reagent kit
NEBNext Ultra II DNA PCR-free Library Prep Kit for Illumina
Fragmentation
Ultrasonic fragmentation (Covaris)
Platform
Illumina NovaSeq 6000
Read type
Paired-end
Read length
51 bp

RNA-seq

Materials and participants
tumor tissue collected from LU99 tumor-bearing mice: total 16 samples
tumor tissue collected from mice with treatment (JDQ443 + TNO155 + alpelisib + ribociclib) / vehicle condition at 3 hours / 24 hours after the last dose: 4 samples each
  • Health status
    Affected
  • Subject count
    24 (Sample)
Disease
human lung cancer (C349)
Sample description
RNAs extracted from tumor tissues
  • Tumor / normal
    Tumor
Cell line
LU99
Experimental method
RNA-seq
Target
N/A
Reagent kit
Illumina Stranded Total RNA Prep, Ligation with Ribo-Zero Plus
Fragmentation
Enzymatic fragmentation
Platform
Illumina NovaSeq 6000
Read type
Paired-end
Read length
50 bp