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Due to system maintenance, the application system, application review by the Data Access Committee will be unavailable during the following period.
Schedule: October 5th (Mon), 2026, 9:00 - October 7th (Wed), 2026, 15:00 (JST)
We apologize for any inconvenience this may cause and appreciate your understanding.

We are currently receiving a large number of applications for data submission, and the review process is taking longer than usual.We sincerely apologize for the delay and kindly ask for your understanding. When submitting an application, we would greatly appreciate it if you could allow sufficient time for the processing.

Following a change to our organizational structure effective April 1, 2026, this division has been renamed from the "Database Center for Life Science, Joint Support-Center for Data Science Research" to the "Database Division for Life Science (DBCLS), BioData Science Initiative (BSI), National Institute of Genetics (NIG)". Where the former name still appears in the guidelines, please read it as the new name.

Dataset ID

JGAD000749

Type of data
NGS (scRNA-seq)
Access criteria
Controlled-access (Type I)
Total data volume
66.7 GB
File formats
  • FASTQ
Research
hum0404
Date published
2025-10-01
Date modified
2025-10-01
DDBJ Search
JGAD000749 (opens in a new tab)
JGA Study
JGAS000620 (opens in a new tab)

Analysis method

scRNA-seq

Materials and participants
cardiomyopathy (ICD10: I42.0): 1 case
GFP or S-RBD-sfGFP was added to iPS cell-derived cardiomyocytes generated from a cardiomyopathy case: 2 samples
  • Health status
    Affected
  • Subject count
    2 (Sample)
Disease
cardiomyopathy (I420)
Sample description
RNAs extracted from iPS cell-derived cardiomyocytes
  • Tissue
    iPSC-derived cardiomyocyte
  • Tumor / normal
    Normal
Sample provider
N/A
Experimental method
scRNA-seq
Target
N/A
Reagent kit
Chromium Next GEM Single Cell 3' v3.1
Fragmentation
included in the above library construction kit
Platform
Illumina HiSeq X
Read type
Paired-end
Read length
91 bp
Reference genome
GRCh38
Mapping
Cell Ranger v7.0.1
QC and filtering
Unique Molecular Identifiers (UMI): 10-17 (Log2) UMIs per Barcode.
Thresholds for genes expressed: more than seven (Log2) genes per barcode.
Thresholds for mitochondrial UMI: Remove the barcodes with more than 20% of mitochondrial UMI counts.
Analysis method
Cell Ranger v7.0.1
Gene count
GFP: 23,234
S-RBD-sfGFP: 23,349
Data use policy
NBDC data sharing policy (JGAP000001)