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Dataset ID

JGAD000740

Type of data
NGS (Amplicon-seq)
Access criteria
Controlled-access (Type I)
Total data volume
54.0 GB
File formats
  • FASTQ
Research
hum0344
Date published
2023-05-10
Date modified
2023-05-10

Analysis method

RNA Amplicon-seq

Materials and participants
Chronic kidney disease, CKD stage G1, G2, G4, G5 (ICD10: N18.1, N18.2, N18.4, N18.5): 77 cases (78 samples)
HEK293 cell line: 1 sample
  • Health status
    Affected
  • Subject count
    77 (Individual)
Disease
G5 (N181, N182, N184, N185)
Sample description
mitochondrial DNAs extracted from non-tumor tissues from chronic kidney disease patients and HEK293 cell line
  • Tissue
    Kidney
  • Tumor / normal
    Normal
Cell line
HEK293
Experimental method
Amplicon-seq
Target
mitochondrial genome
Reagent kit
KAPA Hyper Prep Kit
TruSeq DNA Sample Prep Kit
Fragmentation
Ultrasonic fragmentation (Covaris)
Platform
Illumina HiSeq 2500
Read type
Paired-end
Read length
126 bp