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Dataset ID

JGAD000732

Type of data
NGS (RNA-seq, Target Capture)
Access criteria
Controlled-access (Type I)
Total data volume
79.6 GB
File formats
  • FASTQ
Research
hum0384
Date published
2024-06-20
Date modified
2024-06-20

Unrestricted-access files linked to this dataset

FileLabelSizeCopy URL
DHS-003Z_genelist.xlsxQIAseq DHS-003Z: Human Myeloid Neoplasms Panel7.4 KB

Analysis method

RNA-seq

Materials and participants
AML (ICD10: C92.0): 4 cases
MDS (ICD10: D46): 30 cases
healthy control: 4 samples
  • Health status
    Mixed
  • Subject count
    44 (Mixed)
Disease
AML (C920)
MDS (D46)
Sample description
RNAs extracted from tumor cells and normal cells
  • Tissue
    Bone marrow
  • Tumor / normal
    Mixed
Sample provider
LONZA 2M-101A, 2M-101C: Bone Marrow CD34+ cells
Experimental method
RNA-seq
Target
N/A
Reagent kit
NEBNext Ultra DNA Library Prep Kit for Illumina
SMARTer Ultra Low Input RNA Kit for Sequencing
Fragmentation
Ultrasonic fragmentation
Platform
Illumina HiSeq 1500
Illumina HiSeq 2500
Read type
Single-end
Read length
60 bp

Targeted DNA sequencing

Materials and participants
AML (ICD10: C92.0): 4 cases
MDS (ICD10: D46): 30 cases
  • Health status
    Affected
  • Subject count
    34 (Individual)
Disease
AML (C920)
MDS (D46)
Sample description
DNAs extracted from tumor cells
  • Tumor / normal
    Tumor
Sample provider
N/A
Experimental method
Targeted DNA sequencing
Reagent kit
QIAseq Targeted DNA Panels
Fragmentation
Enzymatic fragmentation
Platform
Illumina MiSeq
Read type
Paired-end
Read length
151 bp