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Following a change to our organizational structure effective April 1, 2026, this division has been renamed from the "Database Center for Life Science, Joint Support-Center for Data Science Research" to the "Database Division for Life Science (DBCLS), BioData Science Initiative (BSI), National Institute of Genetics (NIG)". Where the former name still appears in the guidelines, please read it as the new name.

Dataset ID

JGAD000669

Type of data
NGS (scRNA-seq, RNA-seq, Exome)
Access criteria
Controlled-access (Type I)
Total data volume
172 GB
File formats
  • FASTQ
Research
hum0201
Date published
2022-07-29
Date modified
2022-07-29

Analysis method

WES

Materials and participants
gastrointestinal inflammatory disease (ICD10: K51): 29 cases
gastrointestinal inflammatory disease with neoplastic disease (ICD10: K51, C18, C19, C20): 26 cases
organoids derived from healthy normal colonic tissues with neoplastic disease (ICD10: C18): 2 cases
controls who underwent colonoscopy: 16 individuals
pancreatic cancer (ICD10: C25): 50 cases
(organoids derived from tumor tissues)
  • Health status
    Mixed
  • Subject count
    123 (Individual)
Disease
gastrointestinal inflammatory disease (K51)
gastrointestinal inflammatory disease with neoplastic disease (K51, C18, C19, C20)
neoplastic disease (C18)
pancreatic cancer (C25)
Sample description
DNAs extracted from peripheral blood cells and organoids established from normal or inflammatory/tumor tissues of the patients or controls
  • Tissue
    Colon, Organoid, Peripheral blood
  • Tumor / normal
    Mixed
Sample provider
N/A
Experimental method
WES
Target
N/A
Reagent kit
SureSelect Human All Exon
Fragmentation
Ultrasonic fragmentation
Platform
Illumina HiSeq 2500
Illumina HiSeq 4000
Illumina NovaSeq 6000
Read type
Paired-end
Read length
200 bp

RNA-seq

Materials and participants
Neoplastic disease (ICD10: C18): 2 cases (8 samples)
  • Health status
    Mixed
  • Subject count
    8 (Sample)
Disease
Neoplastic disease (C18)
Sample description
Total RNA extracted from normal duodenum organoids with or without a four-day medium rotation, and after 0, 1, 2 or 4 days of medium rotation
  • Tissue
    Duodenum, Organoid
  • Tumor / normal
    Normal
Sample provider
N/A
Experimental method
RNA-seq
Target
N/A
Reagent kit
TruSeq RNA Library Prep Kit
TruSeq Stranded mRNA Library Prep Kit
Fragmentation
Heat treatment
Platform
Illumina NovaSeq 6000
Read type
Paired-end
Read length
150 bp

scRNA-seq

Materials and participants
Neoplastic disease (ICD10: C16): 1 case (1 sample)
  • Health status
    Mixed
  • Subject count
    1 (Sample)
Disease
Neoplastic disease (C16)
Sample description
RNAs extracted from organoids from normal colonic tissue
  • Tissue
    Colon, Organoid
  • Tumor / normal
    Normal
Sample provider
N/A
Experimental method
scRNA-seq
Target
N/A
Reagent kit
Chromium Single Cell 3' v2
Chromium A Chip Kit
Fragmentation
Enzymatic fragmentation
Platform
Illumina HiSeq 4000
Read type
Paired-end
Read length
read1: 28 bp
read2: 91 bp