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Due to system maintenance, the application system, application review by the Data Access Committee will be unavailable during the following period.
Schedule: October 5th (Mon), 2026, 9:00 - October 7th (Wed), 2026, 15:00 (JST)
We apologize for any inconvenience this may cause and appreciate your understanding.

We are currently receiving a large number of applications for data submission, and the review process is taking longer than usual.We sincerely apologize for the delay and kindly ask for your understanding. When submitting an application, we would greatly appreciate it if you could allow sufficient time for the processing.

Following a change to our organizational structure effective April 1, 2026, this division has been renamed from the "Database Center for Life Science, Joint Support-Center for Data Science Research" to the "Database Division for Life Science (DBCLS), BioData Science Initiative (BSI), National Institute of Genetics (NIG)". Where the former name still appears in the guidelines, please read it as the new name.

Dataset ID

JGAD000621

Type of data
Read count data of miRNA
Access criteria
Controlled-access (Type I)
Total data volume
54.7 KB
File formats
  • TSV
Research
hum0197
Date published
2022-02-21
Date modified
2025-10-29
DDBJ Search
JGAD000621 (opens in a new tab)
JGA Study
JGAS000504 (opens in a new tab)

Analysis method

miRNA-seq

Materials and participants
141 Japanese individuals
  • Subject count
    141 (Individual)
  • Population
    Japanese
Sample description
RNAs extracted from PBMC
  • Tissue
    PBMC, Peripheral blood
  • Tumor / normal
    Normal
Sample provider
N/A
Experimental method
Small RNA-seq
Target
N/A
Reagent kit
SMARTer smRNA-Seq Kit for Illumina
Fragmentation
N/A
Platform
Illumina HiSeq 2500
Read type
Single-end
Read length
100 bp
Reference genome
GRCh37
Mapping
bowtie (GRCh37)
QC and filtering
We performed adapter trimming using Cutadapt v1.8 and removed reads with a low quality score (Phred quality score < 20 in >20% of total bases) using fastp v0.20.0. Also, we removed reads with a length of >29 bp or <15 bp, which are not expected to be mature miRNAs. Mature miRNAs detected with ≥1 read in at least half of the individuals were included in the dataset.
Analysis method
featureCounts + miRbase v22
Gene count
343
Processed data type
miRNA expression count
Data use policy
NBDC data sharing policy (JGAP000001)