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We are currently receiving a large number of applications for data submission, and the review process is taking longer than usual.We sincerely apologize for the delay and kindly ask for your understanding. When submitting an application, we would greatly appreciate it if you could allow sufficient time for the processing.

Following a change to our organizational structure effective April 1, 2026, this division has been renamed from the "Database Center for Life Science, Joint Support-Center for Data Science Research" to the "Database Division for Life Science (DBCLS), BioData Science Initiative (BSI), National Institute of Genetics (NIG)". Where the former name still appears in the guidelines, please read it as the new name.

Dataset ID

JGAD000602

Type of data
B cell receptor repertoire clonotype data from B cell RNA-seq
Access criteria
Controlled-access (Type I)
Total data volume
12.5 GB
File formats
  • TXT
Research
hum0214
Date published
2023-07-10
Date modified
2023-07-10
DDBJ Search
JGAD000602 (opens in a new tab)
JGA Study
JGAS000485 (opens in a new tab)

Analysis method

RNA-seq + BCR-seq

Materials and participants
Systemic Lupus Erythematosus (ICD10: M329): 136 cases (159 samples)
Systemic Sclerosis (ICD10: M340): 90 cases (90 samples)
Myositis (ICD10: M339, M332): 85 cases (85 samples)
Mixed Connective Tissue Disease (ICD10: M351): 21 cases (21 samples)
Sjögren's Syndrome (ICD10: M350): 18 cases (18 samples)
Rheumatoid Arthritis (ICD10: M0690): 27 cases (27 samples)
Behçet's Disease (ICD10: M352): 23 cases (23 samples)
Adult Onset Still's Disease (ICD10: M0610): 18 cases (18 samples)
ANCA-associated Vasculitis (ICD10: M318): 24 cases (24 samples)
Takayasu's Arteritis (ICD10: M314): 16 cases (16 samples)
13 healthy controls (13 samples)
  • Health status
    Healthy
Disease
  • Systemic Lupus Erythematosus
  • Systemic Sclerosis
  • Myositis
  • Mixed Connective Tissue Disease
  • Sjögren's Syndrome
  • Rheumatoid Arthritis
  • Behçet's Disease
  • Adult Onset Still's Disease
  • ANCA-associated Vasculitis
  • Takayasu's Arteritis
Sample description
Total RNAs extracted from 5 B cell subsets (Naive_B, USM_B, SM_B, DN_B, Plasmablast)
  • Tissue
    B cell, Peripheral blood
Sample provider
N/A
Experimental method
BCR-seq
RNA-seq
Target
N/A
Reagent kit
SMART-Seq v4 Ultra Low Input RNA Kit
Fragmentation
SMART-Seq v4 Ultra Low Input RNA Kit
Platform
Illumina HiSeq 2500
Read type
Paired-end
Read length
100 bp
Mapping
MiXCR
QC and filtering
The adaptor sequences and 3' low quality bases (Phred quality score < 20) were trimmed. Short reads (< 50bp) and reads containing many low quality bases (Phred quality score < 20 in > 20% of the bases) were removed.Samples with more than 500 unique CDR-H3 sequences were used for the analysis.
Analysis method
MiXCR
Processed data type
B cell receptor repertoire clonotype data
Data use policy
NBDC data sharing policy (JGAP000001)