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NBDC Human Database

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Dataset ID

JGAD000502

Type of data
NGS (semibulk RNA-seq)
NGS (scRNA-seq)
Access criteria
Controlled-access (Type I)
Total data volume
272 GB
File formats
  • FASTQ
Research
hum0200
Date published
2022-08-23
Date modified
2022-08-25

Analysis method

semibulk RNA-seq

Materials and participants
DCIS (ICD10: D051): 1 case
tumor tissues: 2 libraries from 1 sample
IDC (ICD10: C509): 1 case
tumor tissues: 2 libraries from 1 sample
  • Health status
    Affected
  • Subject count
    2 (Individual)
Disease
IDC (C509)
DCIS (D051)
Sample description
Semibulks (cell clumps) prepared from tumor tissues
  • Tumor / normal
    Tumor
Sample provider
N/A
Experimental method
RNA-seq
Target
N/A
Reagent kit
Drop-seq
Fragmentation
Tagmentation with Nextera XT (Illumina)
Platform
Illumina HiSeq 3000
Illumina NovaSeq 6000
Read type
Paired-end
Read length
200 bp (100PE)

scRNA-seq

Materials and participants
DCIS (ICD10: D051): 1 case
tumor tissues: 1 sample
non-tumor tissues: 1 sample
IDC (ICD10: C509): 1 case
tumor tissues: 1 sample
non-tumor tissues: 1 sample
  • Health status
    Affected
  • Subject count
    2 (Individual)
Disease
IDC (C509)
DCIS (D051)
Sample description
Single cells obtained from tumor and non-tumor tissues
  • Tumor / normal
    Mixed
Sample provider
N/A
Experimental method
scRNA-seq
Target
N/A
Reagent kit
Chromium Single Cell 3' v3
Fragmentation
Enzymatic fragmentation
Platform
Illumina HiSeq 3000
Read type
Paired-end
Read length
124 bp (26/98PE)