Dataset ID
JGAD000492
- Type of data
- NGS (RNA-seq, ChIP-seq)
- Access criteria
- Controlled-access (Type I)
- Total data volume
- 208 GB
- File formats
- FASTQ
- Research
- hum0201
- Date published
- 2021-11-30
- Date modified
- 2022-10-27
- DDBJ Search
- JGAD000492 (opens in a new tab)
- JGA Study
- JGAS000378 (opens in a new tab)
Analysis method
RNA-seq
- Materials and participants
- Neoplastic disease: 23 + 4 cases (35+ 5 samples)
Small cell lung cancer (ICD10: C34)
Biliary tract cancer (ICD10: C23, C24, C78)
Colon cancer (ICD10: C18-20)
Duodenal cancer (ICD10: C17)
Esophageal cancer (ICD10: C15)
Stomach cancer (ICD10: C16, C78)
Liver cancer (ICD10: C22)
Pancreatic cancer (ICD10: C25) - Health statusMixed
- Subject count40 (Sample)
- Disease
- Small cell lung cancer (C34)
Biliary tract cancer (C23, C24, C78)
Colon cancer (C18, C19, C20)
Duodenal cancer (C17)
Esophageal cancer (C15)
Stomach cancer (C16, C78)
Liver cancer (C22)
Pancreatic cancer (C25) - Sample description
- RNAs extracted from organoids established from tumor tissues and a normal epithelial tissue of the patients
RNAs extracted from organoids with BET bromodomain inhibitor established from tumor tissues and a normal epithelial tissue of colon cancer patients - TissueColon, Organoid
- Tumor / normalMixed
- Sample provider
- N/A
- Experimental method
- RNA-seq
- Target
- N/A
- Reagent kit
- TruSeq RNA Library Prep Kit
TruSeq Stranded mRNA Library Prep Kit - Fragmentation
- Heat treatment
- Platform
- Illumina HiSeq 2500
Illumina HiSeq X
Illumina NovaSeq 6000 - Read type
- Paired-end
- Read length
- 250 bp
150 bp - Data use policy
- NBDC data sharing policy (JGAP000001)
ChIP-seq
- Materials and participants
- Neoplastic disease: 4 cases (5 samples)
Colon cancer (ICD10: C18) - Health statusMixed
- Subject count5 (Sample)
- Disease
- Colon cancer (C18)
- Sample description
- DNAs extracted from organoids derived from colon cancer tissues and a normal epithelial tissue of the patients, and immunoprecipitated with an anti-histone antibody (H3K27Ac)
- TissueColon, Organoid
- Tumor / normalMixed
- Sample provider
- N/A
- Experimental method
- ChIP-seq
- Target
- N/A
- Reagent kit
- Illumina Tagment DNA TDE1 Enzyme and Buffer Kits
- Fragmentation
- Ultrasonic fragmentation (Bioruptor Ⅱ)
- Platform
- Illumina HiSeq X
- Read type
- Paired-end
- Read length
- 150 bp
- Data use policy
- NBDC data sharing policy (JGAP000001)