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NBDC HumanDB
JGAD000481
Release date
2021-11-30

Date modified
2021-11-30

Research
hum0300

Type of data
NGS (Exome, RNA-seq)

Access type
Controlled-access (Type I)

JGA Study
JGAS000367

Analysis method

WES

Materials and Participants

Esophageal squamous cell carcinoma (ICD10: C15): 88 cases tumor and paired non-tumor tissue

Experimental Method

Exome

Platform

Illumina [HiSeq 2000]

Sample Description

DNAs extracted from tumor and non-tumor tissues

Library Construction

SureSelect XT Human All Exon

Fragmentation

Ultrasonic fragmentation (Covaris)

Read Type

Paired-end

Read Length

100 bp

Japanese Genotype-phenotype Archive Dataset Accession
Total Data Volume

6 TB (fastq)

Policies

RNA-seq

Materials and Participants

Esophageal squamous cell carcinoma (ICD10: C15): 57 cases (56 cases are also used for Exome) tumor and paired non-tumor tissue

Experimental Method

RNA-seq

Platform

Illumina [HiSeq 2500]

Sample Description

RNAs extracted from tumor and non-tumor tissues

Library Construction

Ribo-Zero Magnetic Gold Kit, TruSeq Stranded mRNA Sample Prep Kit

Fragmentation

TruSeq Stranded mRNA Sample Prep Kit

Read Type

Paired-end

Read Length

100 bp

Japanese Genotype-phenotype Archive Dataset Accession
Total Data Volume

6 TB (fastq)

Policies