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Dataset ID

JGAD000463

Type of data
NGS (RNA-seq: PromethION)
NGS (RNA-seq: NovaSeq 6000)
NGS (WGS: PromethION)
Access criteria
Controlled-access (Type I)
Total data volume
2.3 TB
File formats
  • FASTQ
Research
hum0068
Date published
2021-12-01
Date modified
2022-03-17

Analysis method

WGS

Materials and participants
lung adenocarcinoma (ICD10: C349): 21 + 42 cases (41 + 8 + 81 samples)
tumor tissues: 21 + 4 + 42 samples
normal tissues: 20 + 4 + 39 samples
  • Health status
    Affected
  • Subject count
    63 (Individual)
Disease
lung adenocarcinoma (C349)
Sample description
DNAs extracted from tumor and normal tissues of lung adenocarcinomas
  • Tumor / normal
    Mixed
Sample provider
N/A
Experimental method
WGS
Target
N/A
Reagent kit
Ligation Sequencing Kit (SQK-LSK109)
Fragmentation
N/A
Platform
Oxford Nanopore Technologies PromethION
Read type
Single-end
Read length
N/A

RNA-seq

Materials and participants
lung adenocarcinoma (ICD10: C349): 6 cases (12 samples)
tumor tissues: 6 samples
normal tissues: 6 samples
  • Health status
    Affected
  • Subject count
    6 (Individual)
Disease
lung adenocarcinoma (C349)
Sample description
RNAs extracted from tumor and normal tissues of lung adenocarcinomas
  • Tumor / normal
    Mixed
Sample provider
N/A
Experimental method
RNA-seq
Target
N/A
Reagent kit
Ligation Sequencing Kit (SQK-LSK109)
SMART-Seq v4 Ultra Low Input RNA Kit
Fragmentation
N/A
Platform
Oxford Nanopore Technologies PromethION
Read type
Single-end
Read length
850 bp (Mean)

RNA-seq

Materials and participants
lung adenocarcinoma (ICD10: C349): 6 + 5 + 26 cases (12 + 5 + 26 samples)
tumor tissues: 6 + 5 + 26 samples
normal tissues: 6 samples
  • Health status
    Affected
  • Subject count
    37 (Individual)
Disease
lung adenocarcinoma (C349)
Sample description
RNAs extracted from tumor and normal tissues of lung adenocarcinomas
  • Tumor / normal
    Mixed
Sample provider
N/A
Experimental method
RNA-seq
Target
N/A
Reagent kit
Nextera XT DNA Library Prep Kit
SMART-Seq Stranded Kit
SMART-Seq v4 Ultra Low Input RNA Kit
TruSeq Stranded mRNA Library Prep Kit
TruSeq Stranded Total RNA Library Prep Kit with Ribo-Zero Gold
Fragmentation
Tagmentation (Nextera XT DNA Library Preparation Kit)
Platform
Illumina HiSeq 2000
Illumina NovaSeq 6000
Read type
Paired-end
Read length
300 bp

scDNA-seq

Materials and participants
lung adenocarcinoma (ICD10: C349): 2 cases
tumor tissues: 2 samples
  • Health status
    Affected
  • Subject count
    2 (Individual)
Disease
lung adenocarcinoma (C349)
Sample description
DNAs extracted from single cells in tumor tissues of lung adenocarcinomas
  • Tumor / normal
    Tumor
Sample provider
N/A
Experimental method
scDNA-seq
Target
N/A
Reagent kit
Chromium Single Cell DNA Reagent Kit
Fragmentation
N/A
Platform
Illumina NovaSeq 6000
Read type
Paired-end
Read length
300 bp