Dataset ID
JGAD000423
- Type of data
- NGS (scRNA-seq)
NGS (bulk RNA-seq) - Access criteria
- Controlled-access (Type I)
- Total data volume
- 280 GB
- File formats
- FASTQ
- CSV
- Research
- hum0147
- Date published
- 2022-02-10
- Date modified
- 2022-02-10
- DDBJ Search
- JGAD000423 (opens in a new tab)
- JGA Study
- JGAS000312 (opens in a new tab)
Analysis method
scRNA-seq
- Materials and participants
- 1 healthy donor (STR5)
5 cancer patients
Bladder cancer (ICD10: C67), Colon cancer (ICD10: C20), Kidney cancer (ICD10: C64), Lung cancer (ICD10: C34.3), Ovarian cancer (ICD10: C56) - Health statusMixed
- Subject count6 (Individual)
- Disease
- Bladder cancer (C67)
Colon cancer (C20)
Kidney cancer (C64)
Lung cancer (C343)
Ovarian cancer (C56) - Sample description
- RNAs extracted from T cells of peripheral blood mononuclear cells from a healthy donor and tumor-infiltrating T cells from cancer patients
- TissuePBMC, Peripheral blood, TIL
- Tumor / normalMixed
- Sample provider
- N/A
- Experimental method
- scRNA-seq
- Target
- N/A
- Reagent kit
- BD Rhapsody Targeted mRNA Kit
- Fragmentation
- N/A
- Platform
- Illumina HiSeq 3000
- Read type
- Paired-end
- Read length
- 100 bp
- Data use policy
- NBDC data sharing policy (JGAP000001)
bulk RNA-seq
- Materials and participants
- Kidney cancer (ICD10: C64): 2 cases
T cells of peripheral blood mononuclear cells: 6 fractions each
tumor-infiltrating T cells: 4 fractions each
Lung cancer (ICD10: C34): 3 cases
tumor-infiltrating T cells: 2 fractions each - Health statusAffected
- Subject count5 (Individual)
- Disease
- Kidney cancer (C64)
Lung cancer (C34) - Sample description
- RNAs extracted from T cell fractions of peripheral blood mononuclear cells and tumor-infiltrating T cell fractions
- TissuePBMC, Peripheral blood, TIL
- Tumor / normalMixed
- Sample provider
- N/A
- Experimental method
- RNA-seq
- Target
- N/A
- Reagent kit
- KAPA Hyper Prep Kit
- Fragmentation
- KAPA Frag
- Platform
- Illumina HiSeq 2500
Illumina HiSeq 4000 - Read type
- Paired-end
- Read length
- 100 bp
- Data use policy
- NBDC data sharing policy (JGAP000001)