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Due to system maintenance, the application system, application review by the Data Access Committee will be unavailable during the following period.
Schedule: October 5th (Mon), 2026, 9:00 - October 7th (Wed), 2026, 15:00 (JST)
We apologize for any inconvenience this may cause and appreciate your understanding.

We are currently receiving a large number of applications for data submission, and the review process is taking longer than usual.We sincerely apologize for the delay and kindly ask for your understanding. When submitting an application, we would greatly appreciate it if you could allow sufficient time for the processing.

Following a change to our organizational structure effective April 1, 2026, this division has been renamed from the "Database Center for Life Science, Joint Support-Center for Data Science Research" to the "Database Division for Life Science (DBCLS), BioData Science Initiative (BSI), National Institute of Genetics (NIG)". Where the former name still appears in the guidelines, please read it as the new name.

Dataset ID

JGAD000401

Type of data
NGS (RNA-seq)
Access criteria
Controlled-access (Type I)
Total data volume
63.6 GB
File formats
  • FASTQ
Research
hum0274
Date published
2022-04-14
Date modified
2022-04-14
DDBJ Search
JGAD000401 (opens in a new tab)
JGA Study
JGAS000294 (opens in a new tab)

Analysis method

RNA-seq

Materials and participants
HeLa cells with knockdown (KD) of a target gene: 20 samples
control KD cultured with/without LeptomycinB (LMB: CRM1 inhibitor), nucleus and cytoplasm (duplicate): total 8 samples
NXF1 KD, nucleus and cytoplasm (duplicate): total 4 samples
MTR4 KD cultured with/without LMB, nucleus and cytoplasm (duplicate): total 8 samples
  • Subject count
    20 (Sample)
  • Sex
    Female
Sample description
RNAs extracted from the nucleus or cytoplasm fractions obtained from HeLa cells with knockdown of a target gene
  • Tumor / normal
    Tumor
Cell line
HeLa
Sample provider
HeLa (ATCC)
Experimental method
RNA-seq
Target
N/A
Reagent kit
TruSeq Stranded mRNA Library Prep Kit
Fragmentation
N/A
Platform
Illumina NovaSeq 6000
Read type
Paired-end
Read length
150 bp
Data use policy
NBDC data sharing policy (JGAP000001)