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Due to system maintenance, the application system, application review by the Data Access Committee will be unavailable during the following period.
Schedule: October 5th (Mon), 2026, 9:00 - October 7th (Wed), 2026, 15:00 (JST)
We apologize for any inconvenience this may cause and appreciate your understanding.

We are currently receiving a large number of applications for data submission, and the review process is taking longer than usual.We sincerely apologize for the delay and kindly ask for your understanding. When submitting an application, we would greatly appreciate it if you could allow sufficient time for the processing.

Following a change to our organizational structure effective April 1, 2026, this division has been renamed from the "Database Center for Life Science, Joint Support-Center for Data Science Research" to the "Database Division for Life Science (DBCLS), BioData Science Initiative (BSI), National Institute of Genetics (NIG)". Where the former name still appears in the guidelines, please read it as the new name.

Dataset ID

JGAD000391

Type of data
NGS (Exome)
NGS (RNA-seq)
Access criteria
Controlled-access (Type I)
Total data volume
728 GB
File formats
  • FASTQ
  • BAM
Research
hum0272
Date published
2021-11-26
Date modified
2022-02-07
DDBJ Search
JGAD000391 (opens in a new tab)
JGA Study
JGAS000285 (opens in a new tab)

Analysis method

WES

Materials and participants
melanoma (ICD10: C439): 4 cases
cultured tumor cells established from melanoma patients: 4 cases, 4 + 1 samples
lymph node metastatic lesions: 1 case, 2 samples
  • Health status
    Affected
  • Subject count
    4 (Individual)
Disease
melanoma (C439)
Sample description
DNAs extracted from cultured tumor cells and lymph nodes
  • Tissue
    Cultured cell, Lymph node
  • Tumor / normal
    Mixed
Sample provider
N/A
Experimental method
WES
Target
N/A
Reagent kit
Hybrid Selection
Fragmentation
Captured / Adaptor ligation
Platform
Illumina HiSeq 2500
Read type
Paired-end
Read length
92 bp (Mean)
Reference genome
GRCh38
Data use policy
NBDC data sharing policy (JGAP000001)

RNA-seq

Materials and participants
melanoma (ICD10: C439): 4 cases
cultured tumor cells established from melanoma patients: 4 cases, 4 samples
  • Health status
    Affected
  • Subject count
    4 (Individual)
Disease
melanoma (C439)
Sample description
RNAs extracted from cultured tumor cells
  • Tissue
    Cultured cell
  • Tumor / normal
    Tumor
Sample provider
N/A
Experimental method
RNA-seq
Target
N/A
Reagent kit
Hybrid Selection
Fragmentation
Captured / Adaptor ligation
Platform
Illumina HiSeq 2500
Read type
Paired-end
Read length
100 bp (Mean)
Data use policy
NBDC data sharing policy (JGAP000001)

scRNA-seq + scTCR-seq

Materials and participants
melanoma (ICD10: C439): 3 cases
tumor-infiltrating lymphocyte (TIL): 2 cases, 3 samples
peripheral blood lymphocyte (PBL): 2 cases, 2 samples
lymph node tumor-infiltrating lymphocyte (LN-TIL): 1 case, 2 samples
  • Health status
    Affected
  • Subject count
    3 (Individual)
Disease
melanoma (C439)
Sample description
RNAs extracted from TIL, PBL and LN-TIL
  • Tissue
    Lymphocyte, Peripheral blood, TIL
  • Tumor / normal
    Mixed
Sample provider
N/A
Experimental method
scRNA-seq
TCR-seq
Target
N/A
Reagent kit
Single Cell Immune Profiling Solution Kit
Fragmentation
Hybrid Selection
Platform
Illumina HiSeq 2500
Read type
Paired-end
Read length
100 bp (Mean)
Data use policy
NBDC data sharing policy (JGAP000001)