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Dataset ID

JGAD000386

Type of data
NGS (Iso-seq, CAGE-seq, RNA-seq)
Access criteria
Controlled-access (Type I)
Total data volume
153 GB
File formats
  • FASTQ
Research
hum0268
Date published
2021-04-09
Date modified
2021-04-09

Analysis method

Iso-seq

Materials and participants
Human colon cancer cell line (ICD10: C18): 1 sample
  • Health status
    Affected
  • Subject count
    1 (Sample)
Disease
Human colon cancer cell line (C18)
Sample description
RNAs extracted from cells
  • Tumor / normal
    Tumor
Cell line
COLO 320DM
Sample provider
ATCC (CCL-220)
Experimental method
Iso-seq
Target
N/A
Reagent kit
SMRTbell Template Prep Kit 2.0
Fragmentation
included in the above library construction kit
Platform
PacBio Sequel
Read type
Single-end
Read length
N/A

CAGE-seq

Materials and participants
Human colon cancer cell line (ICD10: C18): 1 sample
  • Health status
    Affected
  • Subject count
    1 (Sample)
Disease
Human colon cancer cell line (C18)
Sample description
RNAs extracted from cells
  • Tumor / normal
    Tumor
Cell line
COLO 320DM
Sample provider
ATCC (CCL-220)
Experimental method
CAGE-seq
Target
N/A
Reagent kit
N/A
Fragmentation
N/A
Platform
Illumina NextSeq
Read type
Single-end
Read length
75 bp

RNA-seq

Materials and participants
Human colon cancer cell line (ICD10: C18): 1 sample
Colon cancer: 6 patients
(6 tumor tissues and 5 non-tumor tissues)
Endometrial cancer (ICD10: C54.9): 5 patients (5 tumor tissues)
  • Health status
    Affected
  • Subject count
    12 (Mixed)
Disease
Human colon cancer cell line (C18)
Endometrial cancer (C549)
Sample description
RNAs extracted from cells
  • Tumor / normal
    Mixed
Cell line
COLO 320DM
Sample provider
ATCC (CCL-220)
Experimental method
RNA-seq
Target
N/A
Reagent kit
TruSeq Stranded mRNA Library Prep Kit
Fragmentation
included in the above library construction kit
Platform
Illumina NovaSeq 6000
Read type
Paired-end
Read length
100 bp