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Dataset ID

JGAD000346

Type of data
NGS (RNA-seq) for high-risk neuroblastoma
NGS (Target Capture) for high-risk neuroblastoma
Access criteria
Controlled-access (Type I)
Total data volume
309 GB
File formats
  • FASTQ
  • BAM
  • TXT
Research
hum0035
Date published
2020-12-21
Date modified
2022-02-16

Unrestricted-access files linked to this dataset

FileLabelSizeCopy URL
380genesregions.xlsxlist of 380 genes & regions13.4 KB

Analysis method

RNA-seq

Materials and participants
30 high-risk neuroblastoma patients (ICD10: C749)
  • Health status
    Affected
  • Subject count
    30 (Individual)
  • Age group
    Child
Disease
high-risk neuroblastoma patients (C749)
Sample description
RNAs extracted from tumor tissues of pancreatoblastoma, hepatoblastoma, pediatric germ cell tumors and neuroblastoma, and HBL cell lines
  • Tumor / normal
    Tumor
Cell line
Hepatoblastoma cell line
Sample provider
N/A
Experimental method
RNA-seq
Target
N/A
Reagent kit
NEBNext Ultra RNA Library Prep Kit for Illumina
TruSeq RNA Library Prep Kit
Fragmentation
included in the above library construction kit
Platform
Illumina HiSeq 2500
Read type
Paired-end
Read length
108 bp
Reference genome
GRCh37

Targeted DNA sequencing

Materials and participants
30 high-risk neuroblastoma patients (ICD10: C749)
  • Health status
    Affected
  • Subject count
    30 (Individual)
  • Age group
    Child
Disease
high-risk neuroblastoma patients (C749)
Sample description
DNAs extracted from tumor tissues of hepatoblastoma, pediatric germ cell tumors and neuroblastoma, and HBL cell lines
  • Tumor / normal
    Tumor
Cell line
Hepatoblastoma cell line
Sample provider
N/A
Experimental method
Targeted DNA sequencing
Target
380 genes or regions associated with paediatric cancers
(list of 380 genes & regions)
Reagent kit
NEBNext Ultra DNA Library Prep Kit for Illumina
Fragmentation
Ultrasonic fragmentation (Covaris E220)
Platform
Illumina HiSeq 2000
Illumina MiSeq
Read type
Paired-end
Read length
100 bp
150 bp
Reference genome
GRCh37