Dataset ID
JGAD000346
- Type of data
- NGS (RNA-seq) for high-risk neuroblastoma
NGS (Target Capture) for high-risk neuroblastoma - Access criteria
- Controlled-access (Type I)
- Total data volume
- 309 GB
- File formats
- FASTQ
- BAM
- TXT
- Research
- hum0035
- Date published
- 2020-12-21
- Date modified
- 2022-02-16
- DDBJ Search
- JGAD000346 (opens in a new tab)
- JGA Study
- JGAS000246 (opens in a new tab)
Unrestricted-access files linked to this dataset
| File | Label | Size | Copy URL |
|---|---|---|---|
| 380genesregions.xlsx | list of 380 genes & regions | 13.4 KB |
Analysis method
RNA-seq
- Materials and participants
- 30 high-risk neuroblastoma patients (ICD10: C749)
- Health statusAffected
- Subject count30 (Individual)
- Age groupChild
- Disease
- high-risk neuroblastoma patients (C749)
- Sample description
- RNAs extracted from tumor tissues of pancreatoblastoma, hepatoblastoma, pediatric germ cell tumors and neuroblastoma, and HBL cell lines
- Tumor / normalTumor
- Cell line
- Hepatoblastoma cell line
- Sample provider
- N/A
- Experimental method
- RNA-seq
- Target
- N/A
- Reagent kit
- NEBNext Ultra RNA Library Prep Kit for Illumina
TruSeq RNA Library Prep Kit - Fragmentation
- included in the above library construction kit
- Platform
- Illumina HiSeq 2500
- Read type
- Paired-end
- Read length
- 108 bp
- Reference genome
- GRCh37
- Data use policy
- NBDC data sharing policy (JGAP000001)
Targeted DNA sequencing
- Materials and participants
- 30 high-risk neuroblastoma patients (ICD10: C749)
- Health statusAffected
- Subject count30 (Individual)
- Age groupChild
- Disease
- high-risk neuroblastoma patients (C749)
- Sample description
- DNAs extracted from tumor tissues of hepatoblastoma, pediatric germ cell tumors and neuroblastoma, and HBL cell lines
- Tumor / normalTumor
- Cell line
- Hepatoblastoma cell line
- Sample provider
- N/A
- Experimental method
- Targeted DNA sequencing
- Target
- 380 genes or regions associated with paediatric cancers
(list of 380 genes & regions) - Reagent kit
- NEBNext Ultra DNA Library Prep Kit for Illumina
- Fragmentation
- Ultrasonic fragmentation (Covaris E220)
- Platform
- Illumina HiSeq 2000
Illumina MiSeq - Read type
- Paired-end
- Read length
- 100 bp
150 bp - Reference genome
- GRCh37
- Data use policy
- NBDC data sharing policy (JGAP000001)