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Dataset ID

JGAD000258

Type of data
NGS (Exome, RNA-seq, Target Capture)
Access criteria
Controlled-access (Type I)
Total data volume
286 GB
File formats
  • FASTQ
  • BAM
Research
hum0153
Date published
2020-09-28
Date modified
2020-11-18

Analysis method

WES

Materials and participants
Dedifferentiated liposarcoma (ICD10: C49): 29 cases
- 29 dedifferentiated components, 8 well-differentiated components, and 29 peripheral blood tissues
TSGCT (ICD10: D21): 10 cases
- 10 tumor tissues and 10 non-tumor tissues
  • Health status
    Affected
  • Subject count
    39 (Individual)
Disease
Dedifferentiated liposarcoma (C49)
TSGCT (D21)
Sample description
DNAs extracted from tumor and non-tumor tissues/peripheral blood cells
  • Tissue
    Peripheral blood
  • Tumor / normal
    Mixed
Sample provider
N/A
Experimental method
WES
Target
N/A
Reagent kit
SureSelect Human All Exon V5
SureSelect Human All Exon V5 + lincRNA
Fragmentation
Ultrasonic fragmentation (Covaris)
Platform
Illumina HiSeq 2500
Read type
Paired-end
Read length
100 bp

RNA-seq

Materials and participants
Dedifferentiated liposarcoma (ICD10: C49): 19 cases
- 19 Dedifferentiated components and 7 well-differentiated components
TSGCT (ICD10: D21): 18 cases
- 18 tumor tissues
  • Health status
    Affected
  • Subject count
    37 (Individual)
Disease
Dedifferentiated liposarcoma (C49)
TSGCT (D21)
Sample description
RNAs extracted from tumor tissues
  • Tumor / normal
    Tumor
Sample provider
N/A
Experimental method
RNA-seq
Target
N/A
Reagent kit
TruSeq RNA Exome
TruSeq Stranded mRNA Library Prep Kit
Fragmentation
Heat treatment
Platform
Illumina HiSeq 2500
Read type
Paired-end
Read length
126 bp

Targeted DNA sequencing

Materials and participants
TSGCT (ICD10: D21): 34 cases
- 34 tumor tissues and 34 non-tumorl tissues
  • Health status
    Affected
  • Subject count
    34 (Individual)
Disease
TSGCT (D21)
Sample description
DNAs extracted from tumor and non-tumor tissues
  • Tumor / normal
    Mixed
Sample provider
N/A
Experimental method
Targeted DNA sequencing
Target
exon regions of CBL gene
Reagent kit
TruSeq Nano DNA Library Prep Kit
Fragmentation
N/A
Platform
Illumina MiSeq
Read type
Paired-end
Read length
300 bp