Dataset ID
JGAD000251
- Type of data
- NGS (WGS): Sequence raw data, Structural Variants data for each sample
- Access criteria
- Controlled-access (Type I)
- Total data volume
- 5.7 TB
- File formats
- FASTQ
- BAM
- VCF
- BEDPE
- Research
- hum0174
- Date published
- 2020-10-07
- Date modified
- 2020-11-27
- DDBJ Search
- JGAD000251 (opens in a new tab)
- JGA Study
- JGAS000173 (opens in a new tab)
Analysis method
WGS
- Materials and participants
- Purified DNA from Japanese-origin B cell lines: 10 samples
- Subject count10 (Sample)
- PopulationJapanese
- Sample description
- Purified DNA from Japanese-origin B cell lines
- TissueB cell
- Cell line
- Japanese B cell line
- Sample provider
- the Health Science Research Resources Bank (HSRRB), the National Institutes of Biomedical Innovation, Health and Nutrition (NIBIOHN)
- Experimental method
- WGS
- Target
- N/A
- Reagent kit
- SMRTbell Library Prep Kit
- Fragmentation
- 1. Megaruptor, g-tube
2. None - Platform
- 10x Genomics Chromium
PacBio Sequel - Read type
- Mixed
- Read length
- 14,000 bp (1.)
151 bp (2.) - Reference genome
- GRCh38
- Mapping
- 1. minimap2
2. longranger by 10X Genomics - QC and filtering
- 1. Qubit, Pulsed-field gel electrophoresis, TapeStation, Bioanalyzer
2. qPCR, Bioanalyzer - Analysis method
- 1. Sniffles
2. longranger by 10X Genomics - Coverage (depth)
- 29x (1.)
19x (2.) - Variant count
- 16,870 (1. /sample)
5100 (2. /sample) - Data use policy
- NBDC data sharing policy (JGAP000001)
WGS
- Materials and participants
- Purified DNA from Japanese-origin B cell liens: 11 samples
- Subject count11 (Sample)
- PopulationJapanese
- Sample description
- Purified DNA from Japanese-origin B cell lines
- TissueB cell
- Sample provider
- the Health Science Research Resources Bank (HSRRB), the National Institutes of Biomedical Innovation, Health and Nutrition (NIBIOHN)
- Experimental method
- WGS
- Target
- N/A
- Reagent kit
- SMRTbell Library Prep Kit
- Fragmentation
- Megaruptor, g-tube
- Platform
- PacBio Sequel
- Read type
- Single-end
- Read length
- 14,000 bp
- Data use policy
- NBDC data sharing policy (JGAP000001)