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Following a change to our organizational structure effective April 1, 2026, this division has been renamed from the "Database Center for Life Science, Joint Support-Center for Data Science Research" to the "Database Division for Life Science (DBCLS), BioData Science Initiative (BSI), National Institute of Genetics (NIG)". Where the former name still appears in the guidelines, please read it as the new name.

Dataset ID

JGAD000251

Type of data
NGS (WGS): Sequence raw data, Structural Variants data for each sample
Access criteria
Controlled-access (Type I)
Total data volume
5.7 TB
File formats
  • FASTQ
  • BAM
  • VCF
  • BEDPE
Research
hum0174
Date published
2020-10-07
Date modified
2020-11-27

Analysis method

WGS

Materials and participants
Purified DNA from Japanese-origin B cell lines: 10 samples
  • Subject count
    10 (Sample)
  • Population
    Japanese
Sample description
Purified DNA from Japanese-origin B cell lines
  • Tissue
    B cell
Cell line
Japanese B cell line
Sample provider
the Health Science Research Resources Bank (HSRRB), the National Institutes of Biomedical Innovation, Health and Nutrition (NIBIOHN)
Experimental method
WGS
Target
N/A
Reagent kit
SMRTbell Library Prep Kit
Fragmentation
1. Megaruptor, g-tube
2. None
Platform
10x Genomics Chromium
PacBio Sequel
Read type
Mixed
Read length
14,000 bp (1.)
151 bp (2.)
Reference genome
GRCh38
Mapping
1. minimap2
2. longranger by 10X Genomics
QC and filtering
1. Qubit, Pulsed-field gel electrophoresis, TapeStation, Bioanalyzer
2. qPCR, Bioanalyzer
Analysis method
1. Sniffles
2. longranger by 10X Genomics
Coverage (depth)
29x (1.)
19x (2.)
Variant count
16,870 (1. /sample)
5100 (2. /sample)

WGS

Materials and participants
Purified DNA from Japanese-origin B cell liens: 11 samples
  • Subject count
    11 (Sample)
  • Population
    Japanese
Sample description
Purified DNA from Japanese-origin B cell lines
  • Tissue
    B cell
Sample provider
the Health Science Research Resources Bank (HSRRB), the National Institutes of Biomedical Innovation, Health and Nutrition (NIBIOHN)
Experimental method
WGS
Target
N/A
Reagent kit
SMRTbell Library Prep Kit
Fragmentation
Megaruptor, g-tube
Platform
PacBio Sequel
Read type
Single-end
Read length
14,000 bp