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Due to system maintenance, the application system, application review by the Data Access Committee will be unavailable during the following period.
Schedule: October 5th (Mon), 2026, 9:00 - October 7th (Wed), 2026, 15:00 (JST)
We apologize for any inconvenience this may cause and appreciate your understanding.

We are currently receiving a large number of applications for data submission, and the review process is taking longer than usual.We sincerely apologize for the delay and kindly ask for your understanding. When submitting an application, we would greatly appreciate it if you could allow sufficient time for the processing.

Following a change to our organizational structure effective April 1, 2026, this division has been renamed from the "Database Center for Life Science, Joint Support-Center for Data Science Research" to the "Database Division for Life Science (DBCLS), BioData Science Initiative (BSI), National Institute of Genetics (NIG)". Where the former name still appears in the guidelines, please read it as the new name.

Dataset ID

JGAD000214

Type of data
NGS (RNA-seq)
NGS (ChIP-seq)
NGS (PBAT-seq)
NGS (ATAC-seq)
Access criteria
Controlled-access (Type I)
Total data volume
1.8 TB
File formats
  • FASTQ
  • BZIP2
Research
hum0147
Date published
2020-09-28
Date modified
2020-11-18
DDBJ Search
JGAD000214 (opens in a new tab)
JGA Study
JGAS000145 (opens in a new tab)

Analysis method

RNA-seq

Materials and participants
2 healthy donors (STR1, STR5)
  • Health status
    Healthy
  • Subject count
    2 (Individual)
Sample description
RNAs extracted from 5 fractions from peripheral blood cells of healthy donors
- Fraction 1: naive Treg
- Fraction 2: effector Treg
- Fraction 3: Foxp3+ effector conventional T cells
- Fraction 5: effector conventional T cells
- Fraction 6: naive T cells
  • Tissue
    Peripheral blood
  • Tumor / normal
    Normal
Sample provider
N/A
Experimental method
RNA-seq
Target
N/A
Reagent kit
KAPA Library Preparation Kit
Fragmentation
KAPA Frag
Platform
Thermo Fisher Scientific Ion S5
Read type
Single-end
Read length
150 bp (Mean)
Data use policy
NBDC data sharing policy (JGAP000001)

ChIP-seq

Materials and participants
1 healthy donor (STR1)
  • Health status
    Healthy
  • Subject count
    1 (Individual)
Sample description
DNAs extracted from 5 fractions from peripheral blood cells of healthy donors, and immunoprecipitated with an anti-histone antibody (H3K27ac)
- Fraction 1: naive Treg
- Fraction 2: effector Treg
- Fraction 3: Foxp3+ effector conventional T cells
- Fraction 5: effector conventional T cells
- Fraction 6: naive T cells
  • Tissue
    Effector T cell, Effector Treg cell, Foxp3+ effector T cell, Naive T cell, Naive Treg cell
  • Tumor / normal
    Normal
Sample provider
N/A
Experimental method
ChIP-seq
Target
N/A
Reagent kit
KAPA Library Preparation Kit
Fragmentation
Branson model1250D
Platform
Thermo Fisher Scientific Ion S5
Read type
Single-end
Read length
150 bp (Mean)
Data use policy
NBDC data sharing policy (JGAP000001)

PBAT-seq

Materials and participants
4 healthy donors (STR1, STR5, STR6, BDR1)
  • Health status
    Healthy
  • Subject count
    4 (Individual)
Sample description
DNAs extracted from 5 fractions from peripheral blood cells of healthy donors
- Fraction 1: naive Treg
- Fraction 2: effector Treg
- Fraction 3: Foxp3+ effector conventional T cells
- Fraction 5: effector conventional T cells
- Fraction 6: naive T cells
  • Tissue
    Effector T cell, Effector Treg cell, Foxp3+ effector T cell, Naive T cell, Naive Treg cell
  • Tumor / normal
    Normal
Sample provider
N/A
Experimental method
PBAT-seq
Target
N/A
Reagent kit
EZ DNA Methylation-Gold Kit
Accel-NGS Methyl-Seq DNA Library Kit
Fragmentation
ACCEL-NGS METHYL-SEQ DNA LIBRARY KIT ver3.0
Platform
Illumina HiSeq 2500
Illumina NovaSeq 6000
Read type
Mixed
Read length
100 bp
150 bp
Reference genome
GRCh38
Data use policy
NBDC data sharing policy (JGAP000001)

ATAC-seq

Materials and participants
2 healty donors (STR1, STR5)
  • Health status
    Healthy
  • Subject count
    2 (Individual)
Sample description
DNAs extracted from 5 fractions from peripheral blood cells of healthy donors
- Fraction 1: naive Treg
- Fraction 2: effector Treg
- Fraction 3: Foxp3+ effector conventional T cells
- Fraction 5: effector conventional T cells
- Fraction 6: naive T cells
  • Tissue
    Effector T cell, Effector Treg cell, Foxp3+ effector T cell, Naive T cell, Naive Treg cell
  • Tumor / normal
    Normal
Sample provider
N/A
Experimental method
ATAC-seq
Target
N/A
Reagent kit
KAPA Library Preparation Kit
Fragmentation
N/A
Platform
Thermo Fisher Scientific Ion S5
Read type
Single-end
Read length
150 bp (Mean)
Data use policy
NBDC data sharing policy (JGAP000001)