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Dataset ID

JGAD000208

Type of data
NGS (scRNA-seq)
Access criteria
Controlled-access (Type I)
Total data volume
48.1 GB
File formats
  • FASTQ
Research
hum0106
Date published
2021-10-27
Date modified
2021-10-27

Analysis method

scRNA-seq

Materials and participants
CRC (ICD10: C18) patients
5 tumor tissues derived from 2 CRC patients (HCT24, HCT25)
(2 cells from HCT24 and 3 cells from HCT25)
non-tumor tissues derived from 1 CRC patient (HCT71)
  • Health status
    Affected
  • Subject count
    6 (Sample)
Disease
CRC (C18)
Sample description
RNAs extracted from single cells of culture tissues derived from CRC patients
  • Tumor / normal
    Mixed
Cell line
Patient-derived cell line
Sample provider
N/A
Experimental method
scRNA-seq
Target
N/A
Reagent kit
Chromium Single Cell 3' v1
Fragmentation
Enzymatic fragmentation
Platform
Illumina HiSeq 2500
Read type
Single-end
Read length
Read1(transcript): 50 bp
Read2(UMI): 15 bp
Index1(CellBarcode): 14 bp
Index2(Sample Index): 8 bp