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Dataset ID

JGAD000089

Type of data
NGS (Exome)
Access criteria
Controlled-access (Type I)
Total data volume
1.2 TB
File formats
  • FASTQ
Research
hum0065
Date published
2020-09-28
Date modified
2020-11-18

Analysis method

WES

Materials and participants
colorectal cancer: 8 cases, lung cancer: 2 cases, small cell carcinoma of uterine cervix: 2case
1. blood cells: 12 samples
(colorectal cancer: 8 samples, lung cancer: 2 samples, small cell carcinoma of uterine cervix: 2samples)
2. CTOS (primary culture): CTOS derived from patient tumors: 5 samples
(colorectal cancer: 2 samples, lung cancer: 2 samples, small cell carcinoma of uterine cervix: 1 sample)
3. CTOS (subculture):
a. CTOS prepared from a CTOS (the same as 2)-derived mouse xenograft: 14 samples
1st subculture: 1 sample (colorectal cancer: 1 sample)
2nd subculture: 4 samples (small cell carcinoma of uterine cervix: 1 sample, colorectal cancer: 3 samples)
3rd subculture: 5 samples (colorectal cancer: 3 samples, lung cancer: 2 samples)
5th subculture: 4 samples (colorectal cancer: 2 samples, lung cancer: 2 samples)
b. CTOS (the same as 2) cultured in vitro: 3 samples (lung cancer: 2 samples, colorectal cancer: 1 sample)
2nd subculture: 1 sample (lung cancer: 1 sample)
5th subculture: 2 samples (lung cancer: 1 sample, colorectal cancer: 1 sample)
  • Health status
    Affected
  • Subject count
    12 (Individual)
Sample description
DNAs extracted from blood cells and CTOSs
  • Tissue
    CTOS, Peripheral blood
  • Tumor / normal
    Mixed
Sample provider
N/A
Experimental method
WES
Target
N/A
Reagent kit
SureSelect Human All Exon V5 + lincRNA
Fragmentation
Ultrasonic fragmentation (Covaris, E220)
Platform
Illumina HiSeq 2000
Read type
Paired-end
Read length
100 bp
Phenotype data
Included