Dataset ID
JGAD000089
- Type of data
- NGS (Exome)
- Access criteria
- Controlled-access (Type I)
- Total data volume
- 1.2 TB
- File formats
- FASTQ
- Research
- hum0065
- Date published
- 2020-09-28
- Date modified
- 2020-11-18
- DDBJ Search
- JGAD000089 (opens in a new tab)
- JGA Study
- JGAS000089 (opens in a new tab)
Analysis method
WES
- Materials and participants
- colorectal cancer: 8 cases, lung cancer: 2 cases, small cell carcinoma of uterine cervix: 2case
1. blood cells: 12 samples
(colorectal cancer: 8 samples, lung cancer: 2 samples, small cell carcinoma of uterine cervix: 2samples)
2. CTOS (primary culture): CTOS derived from patient tumors: 5 samples
(colorectal cancer: 2 samples, lung cancer: 2 samples, small cell carcinoma of uterine cervix: 1 sample)
3. CTOS (subculture):
a. CTOS prepared from a CTOS (the same as 2)-derived mouse xenograft: 14 samples
1st subculture: 1 sample (colorectal cancer: 1 sample)
2nd subculture: 4 samples (small cell carcinoma of uterine cervix: 1 sample, colorectal cancer: 3 samples)
3rd subculture: 5 samples (colorectal cancer: 3 samples, lung cancer: 2 samples)
5th subculture: 4 samples (colorectal cancer: 2 samples, lung cancer: 2 samples)
b. CTOS (the same as 2) cultured in vitro: 3 samples (lung cancer: 2 samples, colorectal cancer: 1 sample)
2nd subculture: 1 sample (lung cancer: 1 sample)
5th subculture: 2 samples (lung cancer: 1 sample, colorectal cancer: 1 sample) - Health statusAffected
- Subject count12 (Individual)
- Sample description
- DNAs extracted from blood cells and CTOSs
- TissueCTOS, Peripheral blood
- Tumor / normalMixed
- Sample provider
- N/A
- Experimental method
- WES
- Target
- N/A
- Reagent kit
- SureSelect Human All Exon V5 + lincRNA
- Fragmentation
- Ultrasonic fragmentation (Covaris, E220)
- Platform
- Illumina HiSeq 2000
- Read type
- Paired-end
- Read length
- 100 bp
- Phenotype data
- Included
- Data use policy
- NBDC data sharing policy (JGAP000001)