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Due to system maintenance, the application system, application review by the Data Access Committee will be unavailable during the following period.
Schedule: October 5th (Mon), 2026, 9:00 - October 7th (Wed), 2026, 15:00 (JST)
We apologize for any inconvenience this may cause and appreciate your understanding.

We are currently receiving a large number of applications for data submission, and the review process is taking longer than usual.We sincerely apologize for the delay and kindly ask for your understanding. When submitting an application, we would greatly appreciate it if you could allow sufficient time for the processing.

Following a change to our organizational structure effective April 1, 2026, this division has been renamed from the "Database Center for Life Science, Joint Support-Center for Data Science Research" to the "Database Division for Life Science (DBCLS), BioData Science Initiative (BSI), National Institute of Genetics (NIG)". Where the former name still appears in the guidelines, please read it as the new name.

Dataset ID

JGAD000085

Type of data
RNA-seq
(Fragments Per Kilobase of exon per Million mapped fragments: FPKM)
Access criteria
Controlled-access (Type I)
Total data volume
56.1 MB
File formats
  • TAR
Research
hum0099
Date published
2020-09-28
Date modified
2025-10-27
DDBJ Search
JGAD000085 (opens in a new tab)
JGA Study
JGAS000085 (opens in a new tab)

Analysis method

RNA-seq

Materials and participants
105 healthy volunteers (21 males, 84 females)
Five immune cell populations (CD4⁺ T cells, CD8⁺ T cells, B cells, NK cells, and monocytes) and whole blood cells (6 samples each)
  • Health status
    Healthy
  • Subject count
    105 (Individual)
  • Sex
    Mixed
Sample description
total RNAs extracted from five immune cell populations (CD4⁺ T cells, CD8⁺ T cells, B cells, NK cells, and monocytes) and whole blood cells
  • Tissue
    B cell, CD4+ T cell, CD8+ T cell, Monocyte, NK cell, Peripheral blood
  • Tumor / normal
    Normal
Sample provider
N/A
Experimental method
RNA-seq
Target
N/A
Reagent kit
TruSeq Stranded mRNA Library Prep Kit
Fragmentation
heat denaturation
Platform
Illumina HiSeq 2500
Read type
Paired-end
Read length
125 bp
Reference genome
GRCh37
Mapping
Tophat2
QC and filtering
Total read counts < 2 x 10⁷ , Mapping rates < 90%, Mean inter-sample correlation coefficients < 0.94
Analysis method
Cufflinks + Gencode version 19 gene model (in FPKM unit)
Read count
4.52 x 10⁷ / 4.39 x 10⁷ on average
Data use policy
NBDC data sharing policy (JGAP000001)