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Dataset ID

JGAD000065

Type of data
NGS (PCR amplicon Seq)
Access criteria
Controlled-access (Type I)
Total data volume
933 MB
File formats
  • FASTQ
Research
hum0068
Date published
2020-09-28
Date modified
2024-10-17

Analysis method

RNA Amplicon-seq

Materials and participants
lung adenocarcinoma (ICD10: C349): 8 cases (7 samples)
  • Health status
    Affected
  • Subject count
    8 (Individual)
Disease
lung adenocarcinoma (C349)
Sample description
cDNA were synthesized from mRNA extracted from lung adenocarcinomas
  • Tissue
    Lung
  • Tumor / normal
    Tumor
Sample provider
N/A
Experimental method
Amplicon-seq
Target
sample 1: Amplicons from EML4-ALK positive case (EML4-ALK region) and KIF5B-RET positive case (KIF5B-RET region) were mixed (1 sample)
sample 2 - 7: Amplicons from 6 cases (EGFR and KRAS regions)
Reagent kit
Nanopore Sequencing Kit
Fragmentation
PCR
Platform
Oxford Nanopore Technologies MinION
Read type
Single-end
Read length
N/A