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Dataset ID

JGAD000061

Type of data
1. Genome & Genetic research
genotype, methylation, gene expression, clinical data for colorectal, gastric, or lung cancer patients.
2. Retrospective study
clinical data for colorectal cancer, gastric cancer, lung cancer, cardiovascular disorders, respiratory dysfunction, diabetes, or nephropathy patients.
Access criteria
Controlled-access (Type I)
Total data volume
41.8 MB
File formats
  • TXT
  • PDF
Research
hum0064
Date published
2020-09-28
Date modified
2020-11-18

Unrestricted-access files linked to this dataset

FileLabelSizeCopy URL
TaqMan_List.xlsx43.7 KB
hum0064_Dictionary_file.xlsx17.1 KB

Analysis method

Sanger sequencing + TaqMan + qRT-PCR + Microarray (薬剤代謝遺伝子 SNP / がん)

Materials and participants
colorectal cancer: 106 cases
gastric cancer: 62 cases
lung cancer: 173 cases
  • Health status
    Affected
  • Subject count
    341 (Individual)
  • Age group
    Mixed
Sample description
-Direct sequencing DNAs extracted from tumors or non-cancerous tissues
-TaqMan analysis DNAs extracted from tumors or non-cancerous tissues
-qRT-PCR RNAs extracted from tumors
-COBRA assay DNAs extracted from tumors
-cDNA microarray RNAs extracted from tumors
  • Tumor / normal
    Mixed
Experimental method
Expression array
qRT-PCR
Sanger sequencing
Target
N/A
Reagent kit
EZ DNA Methylation-Gold Kit
Quick-Amp Labeling Kit
TaqMan SNP Genotyping Assays
ABsolute QPCR Mix
AmpliTaq Gold
Platform
Agilent DNA Microarray Scanner
Agilent Whole Human Genome Microarray 4x44K
Applied Biosystems Genetic Analyzer 3130
Roche LightCycler 480II
Normalization
Refer to JGA000061 experimental support information
Phenotype data
Included

臨床・治療情報

Materials and participants
2-1. Cancer cases
colorectal cancer: 313 cases
gastric cancer: 166 cases
lung cancer: 344 cases

2-2. Non-cancerous cases
diabetes and nephropathy: 300 cases
cardiovascular disorder: 357 cases
respiratory dysfunction: 49 cases
  • Health status
    Mixed
  • Subject count
    1529 (Individual)
  • Age group
    Mixed
Phenotype data
Included