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Dataset ID

E-GEAD-883

Type of data
NGS (RNA-seq)
Access criteria
Unrestricted-access
Total data volume
2.0 MB
File formats
  • TXT
  • CSV
Research
hum0442
Date published
2024-12-06
Date modified
2024-12-06

Analysis method

RNA-seq

Materials and participants
advanced or recurrent non-small cell lung cancer (ICD10: C349) before administering anti-PD-1/PD-L1 antibody treatment: 20 cases
FFPE tumor tissue: 20 samples
  • Health status
    Affected
  • Subject count
    20 (Individual)
Disease
advanced or recurrent non-small cell lung cancer (C349)
Sample description
RNAs extracted from tumor tissues
  • Tumor / normal
    Tumor
Sample provider
N/A
Experimental method
RNA-seq
Target
N/A
Reagent kit
Nextera XT DNA Library Prep Kit
SMART-Seq Stranded Kit
Fragmentation
Nextera XT DNA Library Prep Kit
Platform
Illumina NovaSeq 6000
Read type
Paired-end
Read length
150 bp
Reference genome
GRCh37
GRCh38
Mapping
The count data was mapped to the reference genome using STAR aligner for calculation.
QC and filtering
Read count and TPM data calculated after adapter trimming of fastq files.
Analysis method
HTSeq
Gene count
Count data: 25,559
TPM data: 14,222