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Due to system maintenance, the application system, application review by the Data Access Committee will be unavailable during the following period.
Schedule: October 5th (Mon), 2026, 9:00 - October 7th (Wed), 2026, 15:00 (JST)
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Dataset ID

E-GEAD-867

Type of data
NGS (BD Ab-seq)
Access criteria
Unrestricted-access
Total data volume
438 MB
File formats
  • TXT
Research
hum0416
Date published
2024-09-11
Date modified
2025-01-08
DDBJ Search
E-GEAD-867 (opens in a new tab)

Unrestricted-access files linked to this dataset

Download the URL list
FileLabelSizeCopy URL
Abseq_list.xlsxInformation on the antibodies used for antibody sequencing9.7 KB

Analysis method

CITE-seq (BD Ab-seq)

Materials and participants
microscopic polyangiitis (ICD10: M31.7): 6 cases (4 cases are also used in E-GEAD-635)
7 healthy donors (5 are also used in E-GEAD-635, 2 are also used in DRA019274 / E-GEAD-872)
  • Health status
    Mixed
  • Subject count
    13 (Individual)
Disease
microscopic polyangiitis (M317)
Sample description
RNAs extracted from a single cell isolated from white blood cells
  • Tissue
    PBMC, Peripheral blood
  • Tumor / normal
    Normal
Sample provider
N/A
Experimental method
CITE-seq
Target
Information on the antibodies used for antibody sequencing
Reagent kit
BD Rhapsody WTA Reagent Kit
Fragmentation
Enzymatic Fragmentation
Platform
Illumina HiSeq 2500
Illumina NovaSeq 6000
Read type
Paired-end
Read length
150 bp
Reference genome
GRCh38
QC and filtering
Cell Ranger Metrics
Analysis method
BD Rhapsody Sequence Analysis Pipeline (version 1.12)
Processed data type
Gene expression matrix
Data use policy
NBDC data sharing policy (JGAP000001)