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Due to system maintenance, the application system, application review by the Data Access Committee will be unavailable during the following period.
Schedule: October 5th (Mon), 2026, 9:00 - October 7th (Wed), 2026, 15:00 (JST)
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Dataset ID

E-GEAD-759

Type of data
NGS (RNA-seq) for COVID-19
Protein expression for COVID-19
Access criteria
Unrestricted-access
Total data volume
93.8 MB
File formats
  • TXT
  • TSV
Research
hum0343
Date published
2024-06-28
Date modified
2024-06-28
DDBJ Search
E-GEAD-759 (opens in a new tab)

Unrestricted-access files linked to this dataset

Download the URL list
FileLabelSizeCopy URL
olink-explore-3072-assay-list-2024-03-19.xlsx212 KB

Analysis method

RNA-seq

Materials and participants
COVID-19 (ICD-10: U071): 1,019 cases
  • Health status
    Affected
  • Subject count
    1019 (Individual)
Disease
COVID-19 (U071)
Sample description
RNAs extracted from peripheral blood cells
  • Tissue
    Peripheral blood
  • Tumor / normal
    Normal
Sample provider
N/A
Experimental method
RNA-seq
Target
N/A
Reagent kit
NEBNext Poly(A) mRNA Magnetic Isolation Module
NEBNext Ultra Directional RNA Library Prep Kit for Illumina
Fragmentation
Incubation in the buffer containing Mg2+ at 94°C for 15 minutes
Platform
Illumina NovaSeq 6000
Read type
Paired-end
Read length
100 bp
Reference genome
GRCh38
Mapping
Alignment: STAR (v2.5.3a)
Annotation: GENCODE v30
QC and filtering
Transcripts per kilobase million (TPM) ≥0.1 in ≥20% samples
Read count ≥6 in ≥20% samples
Analysis method
Gene level quantification and normalization: RSEM (v1.3.0)
Gene count
20,329
Data use policy
NBDC data sharing policy (JGAP000001)

Protein expression (2932 proteins)

Materials and participants
COVID-19 (ICD-10: U071): 1,384 cases
  • Health status
    Affected
  • Subject count
    1384 (Individual)
Disease
COVID-19 (U071)
Sample description
Plasma
  • Tissue
    Plasma
  • Tumor / normal
    Normal
Experimental method
Proteomics
Reagent kit
Olink Explore 3072
Platform
Olink Explore 3072
Normalization
Normalized Protein eXpression (NPX) transformation
Bridge sample comparison
Analysis method
OlinkAnalyze v3.4.1
Data use policy
NBDC data sharing policy (JGAP000001)