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Due to system maintenance, the application system, application review by the Data Access Committee will be unavailable during the following period.
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Dataset ID

E-GEAD-628

Type of data
NGS (scRNA-seq)
Access criteria
Unrestricted-access
Total data volume
140 MB
File formats
  • TXT
  • TAR
Research
hum0404
Date published
2023-12-06
Date modified
2023-12-06
DDBJ Search
E-GEAD-628 (opens in a new tab)

Analysis method

scRNA-seq

Materials and participants
cardiomyopathy (ICD10: I42.0): 1 case
GFP or S-RBD-sfGFP was added to iPS cell-derived cardiomyocytes generated from a cardiomyopathy case: 2 samples
  • Health status
    Affected
  • Subject count
    1 (Individual)
Disease
cardiomyopathy (I420)
Sample description
RNAs extracted from iPS cell-derived cardiomyocytes
  • Tissue
    iPSC-derived cardiomyocyte
  • Tumor / normal
    Normal
Sample provider
N/A
Experimental method
scRNA-seq
Target
N/A
Reagent kit
Chromium Next GEM Single Cell 3' v3.1
Fragmentation
included in the above library construction kit
Platform
Illumina HiSeq X
Read type
Paired-end
Read length
91 bp
Reference genome
GRCh38
Mapping
Cell Ranger v7.0.1
QC and filtering
Unique Molecular Identifiers (UMI): 10-17 (Log2) UMIs per Barcode.
Thresholds for genes expressed: more than seven (Log2) genes per barcode.
Thresholds for mitochondrial UMI: Remove the barcodes with more than 20% of mitochondrial UMI counts.
Analysis method
Cell Ranger v7.0.1
Gene count
GFP: 23,234
S-RBD-sfGFP: 23,349
Phenotype data
Included
Data use policy
NBDC data sharing policy (JGAP000001)