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- Release date
- 2023-03-16
- Date modified
- 2023-03-16
- Research
- hum0366
- Type of data
- NGS (RNA-seq) NGS (ATAC-seq)
- Access type
- Unrestricted-access
Analysis method
RNA-seq
- Materials and Participants
healthy subjects: 4 individuals (4 samples each)
- Experimental Method
RNA-seq
- Platform
Illumina [NovaSeq 6000]
- Sample Description
Total RNA extracted from monocytes derived from PBMCs
- Library Construction
Nextera XT DNA Library Preparation Kit
- Fragmentation
Tagmentation (Nextera XT DNA Library Preparation Kit)
- Read Type
Paired-end
- Read Length
101 bp
- Analysis Methods
featureCounts (version 1.6.3)
- Filtering
Low-quality (<20) bases and adaptor sequences were trimmed with Trimmomatic software (version 0.38) with the following parameters: ILLUMINACLIP: path/to/adapter.fa: 2: 30: 10 LEADING: 20 TRAILING: 20 SLIDINGWINDOW: 4: 15 MINLEN: 36.
- Mapping
HISAT2
- Reference Sequence
hg38
- Gene Number
31,706 genes
- Genomic Expression Archive Accession
- Total Data Volume
2.3495 GB (txt)
- Policies
ATAC-seq
- Materials and Participants
healthy subjects: 5 individuals (4 or 5 samples each)
- Experimental Method
ATAC-seq
- Platform
Illumina [NovaSeq 6000]
- Sample Description
DNA extracted from monocytes derived from PBMCs
- Library Construction
ATAC-Seq Kit
- Fragmentation
Tagmentation (ATAC-Seq Kit)
- Read Type
Paired-end
- Read Length
150 bp
- Analysis Methods
featureCounts (version 1.6.3)
- Filtering
Low-quality (<20) bases and adaptor sequences were trimmed with Trimmomatic software (version 0.38) with the following parameters: ILLUMINACLIP: path/to/adapter.fa: 2: 30: 10 LEADING: 20 TRAILING: 20 SLIDINGWINDOW: 4: 15 MINLEN: 36.
- Mapping
Bowtie2
- Reference Sequence
hg38
- Peak Number
33548 peaks
- Genomic Expression Archive Accession
- Total Data Volume
2.3495 GB (txt)
- Policies