Dataset ID
E-GEAD-551
- Type of data
- NGS (scRNA-seq)
- Access criteria
- Unrestricted-access
- Total data volume
- 993 MB
- File formats
- RDS
- Research
- hum0366
- Date published
- 2023-03-20
- Date modified
- 2023-03-20
- DDBJ Search
- E-GEAD-551 (opens in a new tab)
Analysis method
scRNA-seq
- Materials and participants
- COVID-19 (ICD10: U07.1): 16 cases
patients with ARDS: 8 cases
patients without ARDS: 8 cases
healthy subjects : 5 individuals - Health statusMixed
- Subject count21 (Individual)
- Disease
- COVID-19
- Sample description
- RNAs extracted from a single cell isolated from PBMCs
- TissuePBMC, Peripheral blood
- Tumor / normalNormal
- Sample provider
- N/A
- Experimental method
- scRNA-seq
- Target
- N/A
- Reagent kit
- Chromium Next GEM Chip G Single Cell Kit
Chromium Next GEM Single Cell 5' v1.1
Single Index Kit T Set A - Fragmentation
- Enzymatic fragmentation
- Platform
- Illumina NovaSeq 6000
- Read type
- Paired-end
- Read length
- Read1: 26 bp
Read2: 91 bp - Reference genome
- GRCh38
- Mapping
- STAR
- QC and filtering
- Cells with less than 1,000 UMIs or more than 20,000 UMIs, and cells containing more than 10% reads from mitochondrial or hemoglobin genes, were filtered. Furthermore, for each sample, doublets identified by Scrublet were removed.
- Analysis method
- dropEst
- Gene count
- 24,541 genes
- Data use policy
- NBDC data sharing policy (JGAP000001)