We are currently receiving a large number of applications for data submission, and the review process is taking longer than usual.We sincerely apologize for the delay and kindly ask for your understanding. When submitting an application, we would greatly appreciate it if you could allow sufficient time for the processing.
- Release date
- 2023-03-16
- Date modified
- 2023-03-16
- Research
- hum0366
- Type of data
- NGS (scRNA-seq)
- Access type
- Unrestricted-access
Analysis method
scRNA-seq
- Materials and Participants
COVID-19 (ICD10: U07.1): 16 cases patients with ARDS: 8 cases patients without ARDS: 8 cases healthy subjects: 5 individuals
- Experimental Method
scRNA-seq
- Platform
Illumina [NovaSeq 6000]
- Sample Description
RNAs extracted from a single cell isolated from PBMCs
- Library Construction
Chromium Next GEM Single Cell 5′ Library & Gel Bead Kit v1.1, Chromium Next GEM Chip G Single Cell Kit, Single Index Kit T Set A
- Fragmentation
Enzymatic fragmentation
- Read Type
Paired-end
- Read Length
Read1: 26 bp Read2: 91 bp
- Analysis Methods
dropEst
- Filtering
Cells with less than 1,000 UMIs or more than 20,000 UMIs, and cells containing more than 10% reads from mitochondrial or hemoglobin genes, were filtered. Furthermore, for each sample, doublets identified by Scrublet were removed.
- Mapping
STAR
- Reference Sequence
GRCh38
- Gene Number
24,541 genes
- Genomic Expression Archive Accession
- Total Data Volume
2.3495 GB (txt)
- Policies