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Dataset ID

E-GEAD-1107

Type of data
NGS (scRNA-seq, scTCR-seq, scCITE-seq) processed data
Access criteria
Unrestricted-access
Total data volume
355 MB
File formats
  • TXT
  • TAR
Research
hum0511
Date published
2026-07-03
Date modified
2026-08-20

Analysis method

scRNA-seq

Materials and participants
28 healthy individuals (JGAD000957/Dataset 1)
70s-90s: 8
100s: 10
110s: 10
  • Health status
    Healthy
  • Subject count
    34 (Individual)
  • Age group
    Elderly
Sample description
RNAs extracted from peripheral blood T cells
  • Tissue
    Peripheral blood, T cell
  • Tumor / normal
    Normal
Sample provider
N/A
Experimental method
scRNA-seq
Target
N/A
Reagent kit
Chromium Next GEM Single Cell 5' v2
Fragmentation
Enzymatic fragmentation
Platform
MGI DNBSEQ-G400
Read type
Paired-end
Read length
72 bp (Read1)
91 bp (Read2)
Reference genome
GRCh38
Mapping
Cell Ranger 6.1.2
QC and filtering
Cell Ranger 6.1.2
Analysis method
Cell Ranger 6.1.2

scTCR-seq

Materials and participants
28 healthy individuals (JGAD000957/Dataset 1)
70s-90s: 8
100s: 10
110s: 10
  • Health status
    Healthy
  • Subject count
    34 (Individual)
  • Age group
    Elderly
Sample description
RNAs extracted from peripheral blood T cells
  • Tissue
    Peripheral blood, T cell
  • Tumor / normal
    Normal
Sample provider
N/A
Experimental method
TCR-seq
Target
N/A
Reagent kit
Chromium Single Cell V(D)J Enrichment Kit, Human T Cell
Fragmentation
Enzymatic fragmentation
Platform
MGI DNBSEQ-G400
Read type
Paired-end
Read length
72 bp (Read1)
91 bp (Read2)
Reference genome
GRCh38
Mapping
Cell Ranger 6.1.2
QC and filtering
Cell Ranger 6.1.2
Analysis method
Cell Ranger 6.1.2

scCITE-seq

Materials and participants
28 healthy individuals (JGAD000957/Dataset 1)
70s-90s: 8
100s: 10
110s: 10
  • Health status
    Healthy
  • Subject count
    34 (Individual)
  • Age group
    Elderly
Sample description
RNAs extracted from peripheral blood T cells
  • Tissue
    Peripheral blood, T cell
  • Tumor / normal
    Normal
Sample provider
N/A
Experimental method
CITE-seq
Target
CD3, CD4, CD8, CD25, CD27, CD28, CD45RA, CD45RO, CD95, TCRD, CCR7, IL7RA, PD-1, CD161, KLRG1, CD56
Reagent kit
Chromium Single Cell 5' Feature Barcode Library Kit
Fragmentation
Enzymatic fragmentation
Platform
MGI DNBSEQ-G400
Read type
Paired-end
Read length
0 bp (Read1)
15 bp (Read2)
Reference genome
N/A
Mapping
Cell Ranger 6.1.2
QC and filtering
Cell Ranger 6.1.2
Analysis method
Cell Ranger 6.1.2