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NBDC Human Database

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Due to system maintenance, the application system, application review by the Data Access Committee will be unavailable during the following period.
Schedule: October 5th (Mon), 2026, 9:00 - October 7th (Wed), 2026, 15:00 (JST)
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Following a change to our organizational structure effective April 1, 2026, this division has been renamed from the "Database Center for Life Science, Joint Support-Center for Data Science Research" to the "Database Division for Life Science (DBCLS), BioData Science Initiative (BSI), National Institute of Genetics (NIG)". Where the former name still appears in the guidelines, please read it as the new name.

Dataset ID

DRA006296

Type of data
NGS (RNA-seq)
Access criteria
Unrestricted-access
Total data volume
2.2 TB
File formats
  • FASTQ
Research
hum0115
Date published
2019-10-18
Date modified
2020-05-08

Analysis method

RNA-seq

Materials and participants
TF-hESC lines: total 2300 samples
(437 genes x [1 or 2 lines] x [2 or more culture conditions])
  • Subject count
    2300 (Sample)
Sample description
mRNA extracted from TF-hESC cells under each condition
  • Tumor / normal
    Normal
Cell line
hESC
iPSC
Experimental method
RNA-seq
Target
N/A
Reagent kit
SureSelect Strand-Specific RNA Library Prep Kit
Fragmentation
RNA Seq Fragmentation Mix and heat treatment (94℃)
Platform
Illumina HiSeq 2000
Illumina HiSeq 2500
Read type
Mixed
Read length
Single-end: 50 bp
Paired-end: 202 bp
Paired-end: 302 bp