NBDC Research ID: hum0524.v1
SUMMARY
Aims: By conducting genome analysis using NCC Biobank samples, we will clarify the somatic alterations of young-onset and rare cancers and explore therapeutic target molecules.
Methods: Whole exome sequencing, Target capture sequencing, RNA sequencing
Participants/Materials: Ovarian cancer 51 patients (AYA Generation 6 patients, non-AYA Generation 45 patients)
| Dataset ID | Type of Data | Criteria | Release Date |
|---|---|---|---|
| JGAS000831 | Controlled-access (Type I) | 2025/11/11 |
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MOLECULAR DATA
| Participants/Materials |
Ovarian cancer (ICD10: C56) AYA Generation: 6 cases tumor tissues: 6 samples |
| Targets | Exome |
| Target Loci for Capture Methods | - |
| Platform | Illumina [HiSeq 2500] |
| Library Source | DNAs extracted from tumor tissues |
| Cell Lines | - |
| Library Construction (kit name) | SureSelect Human All Exon V5 |
| Fragmentation Methods | Ultrasonic fragmentation (Covaris) |
| Spot Type | Paired-end |
| Read Length (without Barcodes, Adaptors, Primers, and Linkers) | 75 bp |
| Japanese Genotype-phenotype Archive Dataset ID | JGAD000973 |
| Total Data Volume | 702.8 GB (fastq) |
| Comments (Policies) | NBDC policy |
| Participants/Materials |
Ovarian cancer (ICD10: C56) non-AYA Generation: 45 cases tumor tissues: 45 samples |
| Targets | Target Capture |
| Target Loci for Capture Methods |
NCC Oncopanel (114 genes) https://www.ncc.go.jp/en/information/press_release/2019/0717/index.html |
| Platform | Illumina [NextSeq 500] |
| Library Source | DNAs extracted from tumor tissues |
| Cell Lines | - |
| Library Construction (kit name) | NCC Oncopanel kit |
| Fragmentation Methods | Ultrasonic fragmentation (Covaris) |
| Spot Type | Paired-end |
| Read Length (without Barcodes, Adaptors, Primers, and Linkers) | 75 bp |
| Japanese Genotype-phenotype Archive Dataset ID | JGAD000973 |
| Total Data Volume | 702.8 GB (fastq) |
| Comments (Policies) | NBDC policy |
| Participants/Materials |
Ovarian cancer (ICD10: C56) AYA Generation: 6 cases tumor tissues: 6 samples non-AYA Generation: 40 cases tumor tissues : 40 samples |
| Targets | RNA-seq |
| Target Loci for Capture Methods | - |
| Platform | Illumina [NovaSeq 6000] |
| Library Source | RNAs extracted from tumor tissues |
| Cell Lines | - |
| Library Construction (kit name) | Illumina Stranded Total RNA Prep, Ligation with Ribo-Zero Plus |
| Fragmentation Methods | Ultrasonic fragmentation (Covaris) |
| Spot Type | Paired-end |
| Read Length (without Barcodes, Adaptors, Primers, and Linkers) | 150 bp |
| Japanese Genotype-phenotype Archive Dataset ID | JGAD000973 |
| Total Data Volume | 702.8 GB (fastq) |
| Comments (Policies) | NBDC policy |
DATA PROVIDER
Principal Investigator: Takashi Kohno
Affiliation: Research Institute Division of Genome Biology, National Cancer Center
Project / Group Name: Gynecologic oncology in young women
Funds / Grants (Research Project Number):
| Name | Title | Project Number |
|---|---|---|
| National Cancer Center Research and Development Fund | Advancing Therapeutic Research and Drug Discovery through the Evolution and Integration of FIOC Core Facility Functions and Biological Resources | 2020-J-2 |
PUBLICATIONS
| Title | DOI | Dataset ID | |
|---|---|---|---|
| 1 | |||
| 2 |
USRES (Controlled-access Data)
| Principal Investigator | Affiliation | Country/Region | Research Title | Data in Use (Dataset ID) | Period of Data Use |
|---|---|---|---|---|---|
