NBDC Research ID: hum0379.v1
SUMMARY
Aims: Analysis of immune cells in autoimmune diseases
Methods: single-cell RNA+VDJ-seq
Participants/Materials: myasthenia gravis (MG) 2 cases, multiple sclerosis (MS) 4 cases, systemic lupus erythematosus (SLE) 3 cases, 3 healthy cases
| Dataset ID | Type of Data | Criteria | Release Date |
|---|---|---|---|
| JGAS000578 | NGS (scRNA-seq, scVDJ-seq) | Controlled-access (Type I) | 2023/11/20 |
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MOLECULAR DATA
| Participants/Materials |
MG (ICD10: G70.0): 2 cases (CD4+ T cell and B cell specimens each) MS (ICD10: G35): 4 cases (3 cases of CD4+ T cell and B cell specimens each + 1 mixed CD4+ T cell and B cell specimen) SLE (ICD10: M32.9): 3 cases (3 cases of CD4+ T cell and B cell specimens each + 1 CD4+ T cell pecimen) 3 healthy cases (CD4+ T cell and B cell specimens each) |
| Targets | scRNA-seq and scVDJ-seq |
| Target Loci for Capture Methods | - |
| Platform | Illumina [NovaSeq 6000] |
| Library Source | RNAs extracted from CD4+ T cell and B cell isolated from peripheral blood |
| Cell Lines | - |
| Library Construction (kit name) |
scRNA-seq: Chromium Next GEM Single Cell 5’ Kit v2 scVDJ-seq: Chromium Single Cell Human BCR Amplification Kit or Chromium Single Cell Human TCR Amplification Kit |
| Fragmentation Methods | included in the above library construction kit |
| Spot Type | Paired-end |
| Read Length (without Barcodes, Adaptors, Primers, and Linkers) | 91 bp |
| Japanese Genotype-phenotype Archive Dataset ID | JGAD000704 |
| Total Data Volume | 511 GB (fastq) |
| Comments (Policies) | NBDC policy |
DATA PROVIDER
Principal Investigator: Shimon Sakaguchi
Affiliation: Experimental Immunology, IFReC, Osaka University
Project / Group Name: -
| Name | Title | Project Number |
|---|---|---|
| KAKENHI Grant-in-Aid for Specially Promoted Research | Study of the function and development of regulatory T cells | 16H06295 |
| Leading Advanced Projects for medical innovation (LEAP), Advanced Research & Development Programs for Medical Innovation, Japan Agency for Medical Research and Development (AMED) | Study of Immunoregulatory technology targeting regulatory T cells | JP18gm0010005 |
PUBLICATIONS
| Title | DOI | Dataset ID | |
|---|---|---|---|
| 1 | |||
| 2 |
USRES (Controlled-access Data)
| Principal Investigator | Affiliation | Country/Region | Research Title | Data in Use (Dataset ID) | Period of Data Use |
|---|---|---|---|---|---|
