NBDC Research ID: hum0360.v1
SUMMARY
Aims: To understand the mechanism of the pathogenesis of COVID-19 at the single-cell level
Methods: Single-cell analyses were performed using PBMCs from COVID-19 patients.
Participants/Materials: Blood samples from COVID-19 patients
| Dataset ID | Type of Data | Criteria | Release Date |
|---|---|---|---|
| JGAS000546 | NGS (scRNA-seq, scVDJ-seq) | Controlled-access (Type I) | 2022/12/21 |
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MOLECULAR DATA
| Participants/Materials |
COVID-19 (ICD10: U07.1): 6 cases (8 samples) 4 cases: blood samples collected on the day or the next day of admission 2 cases: blood samples collected on the day or the next day of admission + after discharge |
| Targets | scRNA-seq and scVDJ-seq |
| Target Loci for Capture Methods | - |
| Platform | Illumina [NovaSeq 6000] |
| Library Source | RNAs extracted from PBMCs |
| Cell Lines | - |
| Library Construction (kit name) | Chromium Next GEM Single Cell V(D)J Reagent Kits |
| Fragmentation Methods | Enzymatic fragmentation |
| Spot Type | Paired-end |
| Read Length (without Barcodes, Adaptors, Primers, and Linkers) |
scRNA-seq: 91 bp scVDJ-seq: 91 bp, 90 bp |
| Japanese Genotype-phenotype Archive Dataset ID | JGAD000665 |
| Total Data Volume | 342.2 GB (fastq) |
| Comments (Policies) | NBDC policy |
DATA PROVIDER
Principal Investigator: Ai Tachikawa
Affiliation: AIDS Research Center, National Institute of Infectious Diseases
Project / Group Name: -
| Name | Title | Project Number |
|---|---|---|
| Research Program on Emerging and Re-emerging Infectious Diseases, Japan Agency for Medical Research and Development (AMED) | Comprehensive survey of infectious microorganisms and the investigation of host responses against microorganism infections | JP22fk0108146 |
| Research Program on Emerging and Re-emerging Infectious Diseases, Japan Agency for Medical Research and Development (AMED) | Development of pathological diagnostic methods for congenital infections and unexplained illness of possible infectious etiology | JP19fk0108104 |
PUBLICATIONS
| Title | DOI | Dataset ID | |
|---|---|---|---|
| 1 | Multimodal single-cell analyses of peripheral blood mononuclear cells of COVID-19 patients in Japan | doi: 10.1038/s41598-023-28696-9 | JGAD000665 |
| 2 |
USRES (Controlled-access Data)
| Principal Investigator | Affiliation | Country/Region | Research Title | Data in Use (Dataset ID) | Period of Data Use |
|---|---|---|---|---|---|
