NBDC Research ID: hum0337.v1

SUMMARY

Aims: To investigate the characteristics of squamous cell carcinoma arising from mature teratoma of the ovary by single nucleus RNA sequencing.

Methods: Nuclei were isolated from archival fresh frozen cancer tissues and libraries were acquired with the Chromium Next GEM Single Cell 3' Reagent Kits v3.1 following the manufacturer’s protocol (10X Genomics). Then, libraries were sequenced on a Novaseq (Illumina).

Participants/Materials: Five patients with squamous cell carcinoma arising from mature teratoma of the ovary

Dataset ID	Type of Data	Criteria	Release Date
JGAS000521	NGS (scRNA-seq)	Controlled-access (Type I)	2024/07/19

*Release Note

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MOLECULAR DATA

JGAS000521


Participants/Materials	squamous cell carcinoma arising from mature teratoma of the ovary (ICD10: C56): 5 cases tumor tissue: 5 samples
Targets	scRNA-seq
Target Loci for Capture Methods	-
Platform	Illumina [NovaSeq 6000]
Library Source	RNAs extracted from tumor tissues
Cell Lines	-
Library Construction (kit name)	Chromium Next GEM Single Cell 3' Reagent Kits v3.1
Fragmentation Methods	Enzymatic Fragmentation
Spot Type	Paired-end
Read Length (without Barcodes, Adaptors, Primers, and Linkers)	119 bp
Japanese Genotype-phenotype Archive Dataset ID	JGAD000639
Total Data Volume	149 GB (fastq)
Comments (Policies)	NBDC policy

DATA PROVIDER

Principal Investigator: Kosuke Yoshida

Affiliation: Nagoya University Graduate School of Medicine

Project / Group Name: -

Funds / Grants (Research Project Number):

Name	Title	Project Number
KAKENHI Grant-in-Aid for Scientific Research on Innovative Areas	Platform for Advanced Genome Sciences	16H06279

PUBLICATIONS

	Title	DOI	Dataset ID
1
2

USRES (Controlled-access Data)

Principal Investigator	Affiliation	Research Title	Data in Use (Dataset ID)	Period of Data Use