NBDC Research ID: hum0307.v1

SUMMARY

Aims: Evaluate the differences of sebum RNA between healthy males and male patients with atopic dermatitis using the data of study 1 (Analysis for skin molecules and properties in healthy subjects) and study 2 (Analysis for skin molecules in patients with atopic dermatitis).

Methods: AmpliSeq Transcriptome analysis was performed to analyze human mRNA expressions in sebum RNA

Participants/Materials: Healthy males: 29, Male patients with atopic dermatitis: 30

Dataset ID	Type of Data	Criteria	Release Date
JGAS000416	NGS (RNA-seq)	Controlled-access (Type I)	2022/03/11

* Release Note

* Data users need to apply an application for Using NBDC Human Data to reach the Controlled-access Data. Learn more

MOLECULAR DATA

JGAS000416


Participants/Materials	healthy control: 29 males atopic dermatitis (ICD10: L20): 30 males
Targets	RNA-seq
Target Loci for Capture Methods	-
Platform	Thermo Fisher Scientific [Ion S5]
Library Source	mRNAs extracted from sebum
Cell Lines	-
Library Construction (kit name)	Ion AmpliSeq Transcriptome Human Gene Expression Kit
Fragmentation Methods	-
Spot Type	Single-end
Read Length (without Barcodes, Adaptors, Primers, and Linkers)	110 bp
Japanese Genotype-phenotype Archive Dataset ID	JGAD000533
Total Data Volume	261 GB (bam)
Comments (Policies)	NBDC policy

DATA PROVIDER

Principal Investigator: Takayoshi Inoue

Affiliation: Biological Science Laboratories, Kao Corporation

Project / Group Name: Sebum RNA project

Funds / Grants (Research Project Number): -

Name	Title	Project Number
-	-	-

PUBLICATIONS

	Title	DOI	Dataset ID
1	Non-invasive human skin transcriptome analysis using mRNA in skin surface lipids	doi: 10.1038/s42003-022-03154-w	JGAD000533
2

USRES (Controlled-access Data)

Principal Investigator	Affiliation	Country/Region	Research Title	Data in Use (Dataset ID)	Period of Data Use
Michiaki Hamada	Faculty of Science and Engineering, Waseda University	Japan	Construction of RNA-targeted Drug Discovery Database	JGAD000533	2022/12/26-2025/03/31