NBDC Research ID: hum0270.v1
SUMMARY
Aims: To identify prognosis-related genomic alterations in pediatric Osteosarcoma patients using NGS technique.
Methods: By using a comprehensive cancer panel which amplifies coding exons of cancer-related 409 genes, candidate mutations occurred in OS were surveyed.
Participants/Materials: Pediatric Osteosarcoma: 16 patients
| Dataset ID | Type of Data | Criteria | Release Date |
|---|---|---|---|
| JGAS000282 | NGS (Target Capture) | Controlled-access (Type I) | 2022/05/19 |
*Data users need to apply an application for Using NBDC Human Data to reach the Controlled-access Data. Learn more
MOLECULAR DATA
| Participants/Materials | Pediatric Osteosarcoma (ICD10: C41.9): 16 cases |
| Targets | Target Capture |
| Target Loci for Capture Methods | 409 genes (Ion Ampliseq Comprehensive Cancer Panel) |
| Platform | Thermo Fisher Scientific [Ion Torrent Proton] |
| Library Source | DNAs extracted from tumor tissues and non-tumor tissues (or peripheral blood cells) |
| Cell Lines | - |
| Library Construction (kit name) | Ion AmpliSeq™ Library Kit 2.0 |
| Fragmentation Methods | - |
| Spot Type | Single-end |
| Read Length (without Barcodes, Adaptors, Primers, and Linkers) | 155 bp on average |
| Japanese Genotype-phenotype Archive Dataset ID | JGAD000388 |
| Total Data Volume | 131.3 GB（fastq） |
| Comments (Policies) | NBDC policy |
DATA PROVIDER
Principal Investigator: Tsukasa Yonemoto
Affiliation: Division of Orthopedic Surgery, Chiba Cancer Center
Project / Group Name: -
Funds / Grants (Research Project Number):
| Name | Title | Project Number |
|---|---|---|
| KAKENHI Grant-in-Aid for Young Scientists (B) | Molecular profiling to predict chemosensitivity for osteosarcoma | 23791673 |
| KAKENHI Grant-in-Aid for Scientific Research (C) | Analysis of factors determining chemosensitivity and pulmonary metastasis in patients with osteosarcoma by genomic expression analysis | 19591742 |
| Grants-in-Aid by the Ministry of Health, Labor and Welfare of Japan for the Third Term Comprehensive 10-year Strategy for Cancer Control | Genomic and gene expression profiling of pediatric solid tumors |
PUBLICATIONS
| Title | DOI | Dataset ID | |
|---|---|---|---|
| 1 | CDK4 overexpression is a predictive biomarker for resistance to conventional chemotherapy in patients with osteosarcoma | doi: 10.3892/or.2021.8086 | JGAD000388 |
| 2 |
USRES (Controlled-access Data)
| Principal Investigator | Affiliation | Country/Region | Research Title | Data in Use (Dataset ID) | Period of Data Use |
|---|---|---|---|---|---|
