NBDC Research ID: hum0263.v1

 

SUMMARY

Aims: To develop a simple and robust methylation analysis method for risk stratification of patients with juvenile myelomonocytic Leukemia.

Methods: the DREAM method; a method for whole genome methylation analysis using NGS

Participants/Materials: Juvenile myelomonocytic Leukemia

 

Dataset IDType of DataCriteriaRelease Date
JGAS000292 NGS (DREAM-seq) Controlled-access (Type I) 2023/07/24

*Release Note

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MOLECULAR DATA

JGAS000292
Participants/Materials

Juvenile myelomonocytic Leukemia (ICD10: C933): 149 cases (167 samples)

    16 cases (34 samples) were repeatedly examined
Targets DREAM-seq
Target Loci for Capture Methods -
Platform Illumina [HiSeq 2500]
Library Source DNAs extracted from tumor tissues
Cell Lines -
Fragmentation Methods Enzymatic fragmentation
Library Construction (kit name) NEBNext Ultra II DNA Library Prep Kit for Illumina
Spot Type Paired-end
Read Length (without Barcodes, Adaptors, Primers, and Linkers) 150bp
Japanese Genotype-phenotype Archive Dataset ID JGAD000398
Total Data Volume 513.6 GB (fastq)
Comments (Policies) NBDC policy

 

DATA PROVIDER

Principal Investigator: Yoshiyuki Takahashi

Affiliation: Department of Pediatrics, Nagoya University Graduate School of Medicine

Project / Group Name: -

Funds / Grants (Research Project Number):

NameTitleProject Number
KAKENHI Grant-in-Aid for Scientific Research (C) Development of a methylation analysis method for juvenile myelomonocytic leukemia using next-generation sequencing 18K07816

 

PUBLICATIONS

TitleDOIDataset ID
1 Simple and robust methylation test for risk stratification of patients with juvenile myelomonocytic leukemia doi: 10.1182/bloodadvances.2021005080 JGAD000398
2

 

USRES (Controlled-access Data)

Principal Investigator: Affiliation: Data in Use (Dataset ID)Period of Data Use